User:Alexander S Mikheyev/Notebook/oil flies/2009/12/13

Oil flies

Main project page Previous entry

PCR of oil fly bacteria reagents 1x 15x water 6.1 91.5 5x 7.5 112.5 1046R 0.3 4.5 917F 0.3 2.5 U/ul Platinum HiFi Pol 0.3 4.5 template 0.5 total 15 DNA 917F DNA 917F pit91 guts TCGAG pit91 skin ACTCG pit91 guts TCGAG pit91 skin ACTCG pit91 guts TCGAG pit91 skin ACTCG small pit guts CACAC small pit skin ATACG small pit guts CACAC small pit skin ATACG small pit guts CACAC small pit skin ATACG negative all 917F 94C 3 min (94C 10s; 57C 45s; 72C 1m)x 20 cycles