User:Alexander S Mikheyev/Notebook/mutation accumulation in rotifers/2009/12/04
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 Rotifer mutation accumulation
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 Main project page Previous entry
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Test InstaGene Extraction Quality
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| Plate | Well | Genus | 12/2/2009 Count | ' | Reagents | Concentration | 1X | 15X |
| Adenita 23Nov09 | B3 | Adineta | 30+ | H20 | 4.75 | 71.25 | ||
| AV 10-06-09 | well 6 | Adineta | 100+ | 10X Buffer | 10X | 1 | 15 | |
| Boston | A1 | Adineta | 10+ | dye | 10X | 1 | 15 | |
| Boston | A4 | Adineta | 50+ | dNTP | 10mM | 0.2 | 3 | |
| CC | A1 | Adineta | subs 100+ | primer mix | 5uM | 1 | 15 | |
| CC | A3 | Adineta | subs 100+ | Taq | 5U/uM | 0.05 | 0.75 | |
| WH1 | B3 | Adineta | 20+ | DNA | variable | 2 | ||
| WH1 | B5 | Adineta | 20+ | TOTAL | 10 | 120 | ||
| WH1 | B6 | Adineta | 50+ | Cocktail per well = 8.0ul | ||||
| WH1 | C4 | Adineta | 30+ | |||||
| WH2 | A6 | Adineta | 10+ | Primer | HIS F1+R1 | |||
| WH3 | A5 | Adineta | 20+ | |||||
| WH3 | C2 | Adineta | 7 | PCR | 95C | 3 min | ||
| 95C | 10 sec | |||||||
| EXTRACTION | 60C | 2 min | ||||||
| 5-10 individuals | 35 cycles | |||||||
| InstaGene Matrix | 200ul per sample | |||||||
| 56C | 30 min | |||||||
| 99.9C | 8min | |||||||
