User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/08

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Library preparation Reaction mixture conc. 1x Callow (after bisulfite conversion) ?? 1 PCR primer PE 1.0 ?? 0.2 PCR primer PE 2.0 ?? 0.2 Phusion DNA polymerase 2x 5 MilliQ 3.6 total 10 98C 30 sec 98C 10 sec 65C 30 sec 72C 30 sec 25 cycle 72C 5 min load 2.5ul of sample