User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/08

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Library preparation

Reaction mixture conc. 1x
Callow (after bisulfite conversion)??1
PCR primer PE 1.0??0.2
PCR primer PE 2.0??0.2
Phusion DNA polymerase2x5
MilliQ3.6
total10
98C30 sec
98C10 sec
65C30 sec
72C30 sec
25 cycle
72C5 min

load 2.5ul of sample

image:0067-1.jpg



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