User:Alexander S Mikheyev/Notebook/P. rapida partial genome/2009/12/07

P.rapida genome	Main project page Previous entry
QC ligation of the clones water 3.3 buffer 0.5 dntp 0.1 taq 0.1 dna 1 45min @ 72C, proceed to ligation reagent volume conc molarity 2x 1.666666667 vector 0.333333333 50 ng/ul 26 fmol/ul pcr 1 6 ng/ul 61 fmol/ul t4 0.333333333 3U/ul total 3.333333333 same reacti ligation for 4 hours at 16C