User:Alexander S Mikheyev/Notebook/P. rapida partial genome/2009/11/12

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Blunt end repair of P.rapida DNA

Reagents 1x conc.
DNA9060ng/ul
10x12.610x
ATP12.610 mM
[NEB blunting enzyme]2.7
dNTPs12.610 mM
Hold 16 hours at 16C deactivate 10 min @65Cprogram exp003 on PCR
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