User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/25

Biomaterials Design Lab: Spring 2014

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Objective Spin down samples of 30:1 lysozyme-AuNP with 1M salts added and run UV-Vis. Heat samples 30:1 lysozyme-AuNP with 1M salts at 37°C overnight. Image results of 30:1, 55:1, and 110:1 lysozyme-AuNPs synthesized with salt added before heating using UV-Vis. Run atomic absorption of 30:1, 55:1, and 110:1 lysozyme-AuNPs synthesized with salts added before heating. Procedure Atomic Absorption Creating the Gold Stock Solutions Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. Atomic Absorption Samples Solutions with the following Au:lysozyme ratio were run on the AA: 30:1 MgCl2, CaCl2, NaCl, KCl 55:1 MgCl2, CaCl2, NaCl, KCl 110:1 MgCl2, CaCl2, NaCl, KCl Images Atomic Absorance of Lysozyme with KCl, NaCl, CaCl2, MgCl2 Figure 1. Gold:protein Ratio to Concentration of Lysozyme-AuNP Baked with Salts UV-Vis UV-Vis For Solutions that Contained Salt Before Lysozyme AuNP Synthesis UV-Vis For Select Variables at More Refined Concentrations Please Note that the Control Wasn't Diluted With Variable Like the Other Samples. Based off of all UV-Vis data: The ABS for the controls were corrected for the dilution factor present and the percent AuNP's in solution was calculated. Notes These results detail the samples from last Thursday with the addition of the variables to 1 mL aliquots of 30:1 ratio with salts added after. These results detail the reaction of the different ratios synthesized with salts added before heating.