User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/11/06

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Objective

  • Synthesize more Lysozyme linked AuNP's and Hemoglobin linked AuNP's.

Procedure

Stock Solution Prep

  • Gold- .011 grams was added to a 10 ml volumetric flask and was filled to 10 ml with water.
  • Hemoglobin- .0032 grams of hemoglobin was added to a 10 ml volumetric flask and was filled to 10 ml with water.
  • Lysozyme- .02 grams of lysozyme was added to a 25 ml volumetric flask and was filled to 25 ml with water.
    • Specific concentrations are expressed below.

Sample Prep

  • AuNP's were made as dictated below.

Image:Nov_6_solution_cheat_sheet.png

  • Once the solutions were mixed they were heated for 4 hours at 80 degrees Celsius.

Data

  • No data today!
  • Note after the samples were made, all of the hemoglobin AUNP's were clear with fibers except for the smallest ratio that was pink.
  • All of the lysozyme AuNP's made today were pink except ratios higher than 30:1 had fibers.


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