Objective
- To complete the procedure outlined by Dr.Hartings, HERE.
- Synthesize HRP-Nanoparticles for future study.
Procedure
- Specifically we made four different samples of HRP-AuNP's.
- Samples were prepared as follows,
- 30:1 (850ul of .25mM Au solution and 5.11ml of 8.33uM HRP) These were then diluted to 10 ml in volumetric flask.
- 60:1 (850ul of .25mM Au solution and 2.56ml of 4.17uM HRP) These were then diluted to 10 ml in volumetric flask.
- 90:1 (850ul of .25mM Au solution and 1.71ml of 2.78uM HRP) These were then diluted to 10 ml in volumetric flask.
- 120:1 (850ul of .25mM Au solution and 1.28ml of 2.08uM HRP) These were then diluted to 10 ml in volumetric flask.
- Please note that all samples were diluted to 10ml with water.
- All samples were baked at 80 degrees Celsius for 3 hours in the oven.
Data
- No data today, the nanoparticles will be analyzed on another day.
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