User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/11

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Objective

  • To run atomic absorption spectra for gold solution standards as dictated by Dr.Hartings here.
  • Form a calibration curve with the class data to use for future reference.
  • Calculate Concentration of Gold in Citrate Au-NP's and BSA Au-NP's.
  • Calculate the number of gold particles in each sample of Citrate Au-NP's and BSA Au-NP's.

Procedure

  • Samples were prepared as dictated by the table below.

Image:Screen_shot_2013-09-15_at_12.57.28_PM.png

  • Please note that all samples were measured using a 100-microliter pipette and a 1000-microliter pipette.

Data

Individual and Group Calibration Curve

  • The table below represents the results from the atomic absorption spectra. Please note that the max absorbance of gold was 248 nm.
  • Also note that the Citrate Au-NP had to be diluted in order view on AAS. Further calculations must reflect this dilution.

Image:Screen_shot_2013-09-15_at_1.26.23_PM.png

  • Absorbance had to be corrected in order to reflect the non-zero water absorbance.

Image:Screen_shot_2013-09-15_at_2.20.59_PM.png Image:Sept_11_Class_Gold_AAS_Exper_Biochem.png

  • Note individual data is on the left and the class data is on the right.

Grubbs Test Analysis of Group Data

Image:Screen_shot_2013-09-15_at_2.01.10_PM.png

  • Based on the Grubbs test, and using a value of n=5 for each concentration, no data point had a Grubbs value greater than 1.67 using 95% confidence.
  • Do to this fact none of the data points are outliers.

Calculation of Concentration of Gold in Citrate Au-NP's and BSA Au-NP's

  • Note that the best fit of the calibration curve is a polynomial.
  • Using this best fit and the calculated absorbances of the Au-NP's the concentration of gold are the following,
  • Concentration of Gold in Citrate Au-NP's = 74.45 ug/ml (This takes into account the dilution).
  • Concentration of Gold in BSA Au-NP's = 38.35 ug/ml

Calculation of the # of Gold Particles in each Au-NP

  • Volume of BSA Au-NP = 2.809ml
    • Using the concentrations calculated using the AAS the amount of gold in the whole solution is, .00000106 moles.
    • Using avogadros number their is, 6.383 * 10E17 atoms of gold in the BSA Au-NP solution
  • Volume of Citrate Au-NP = 25.6 ml
    • Using the concentrations calculated using the AAS the amount of gold in the whole solution is, .00000498 moles
    • Using avogadros number their is, 2.999 * 10E18 atoms of gold in the citrate Au-NP solution.
    • Know the concentration of the nanoparticles in solution is 8.43 nM


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