User:Adam Rodaway

Contact Info

Adam Rodaway (an artistic interpretation)

• Adam Rodaway
• King's College London
• Cardiovascular Division
• New Hunt's House
• Guy's Campus
• London SE1 1UL, UK
• Email me through OpenWetWare

I work in the Rodaway Lab at King's College London. I learned about OpenWetWare from Google search (I was looking for some E. coli genotypes), and I've joined because I've got some PCR tips and a (better and cheaper than ' Quikchange') site-directed mutagenesis protocol I'd like to share..

Education

• 1992, PhD, Imperial Cancer Research Fund and Imperial College London UK
• 1987, B.A. (Zoology), Cambridge University, UK

Research interests

1. The embryonic development of the blood and vasculature. To study this I use the zebrafish (Danio rerio)
2. Transposon based techniques for transgenesis
3. Using recombination in E. coli to make recombinant molecules: 'recombineering' and recombinational cloning

Publications

1. Liu F, Walmsley M, Rodaway A, and Patient R. Fli1 acts at the top of the transcriptional network driving blood and endothelial development. Curr Biol. 2008 Aug 26;18(16):1234-40. DOI:10.1016/j.cub.2008.07.048 |
2. Reijntjes S, Rodaway A, and Maden M. The retinoic acid metabolising gene, CYP26B1, patterns the cartilaginous cranial neural crest in zebrafish. Int J Dev Biol. 2007;51(5):351-60. DOI:10.1387/ijdb.062258sr |
3. Zhang XY and Rodaway AR. SCL-GFP transgenic zebrafish: in vivo imaging of blood and endothelial development and identification of the initial site of definitive hematopoiesis. Dev Biol. 2007 Jul 15;307(2):179-94. DOI:10.1016/j.ydbio.2007.04.002 |
4. Rodaway A and Patient R. Mesendoderm. an ancient germ layer?. Cell. 2001 Apr 20;105(2):169-72. DOI:10.1016/s0092-8674(01)00307-5 |
5. Weber H, Symes CE, Walmsley ME, Rodaway AR, and Patient RK. A role for GATA5 in Xenopus endoderm specification. Development. 2000 Oct;127(20):4345-60. DOI:10.1242/dev.127.20.4345 |
6. Brown LA, Rodaway AR, Schilling TF, Jowett T, Ingham PW, Patient RK, and Sharrocks AD. Insights into early vasculogenesis revealed by expression of the ETS-domain transcription factor Fli-1 in wild-type and mutant zebrafish embryos. Mech Dev. 2000 Feb;90(2):237-52. DOI:10.1016/s0925-4773(99)00256-7 |
7. Reiter JF, Alexander J, Rodaway A, Yelon D, Patient R, Holder N, and Stainier DY. Gata5 is required for the development of the heart and endoderm in zebrafish. Genes Dev. 1999 Nov 15;13(22):2983-95. DOI:10.1101/gad.13.22.2983 |
8. Rodaway A, Takeda H, Koshida S, Broadbent J, Price B, Smith JC, Patient R, and Holder N. Induction of the mesendoderm in the zebrafish germ ring by yolk cell-derived TGF-beta family signals and discrimination of mesoderm and endoderm by FGF. Development. 1999 Jun;126(14):3067-78. DOI:10.1242/dev.126.14.3067 |
9. Sykes TG, Rodaway AR, Walmsley ME, and Patient RK. Suppression of GATA factor activity causes axis duplication in Xenopus. Development. 1998 Dec;125(23):4595-605. DOI:10.1242/dev.125.23.4595 |
10. Read EM, Rodaway AR, Neave B, Brandon N, Holder N, Patient RK, and Walmsley ME. Evidence for non-axial A/P patterning in the nonneural ectoderm of Xenopus and zebrafish pregastrula embryos. Int J Dev Biol. 1998 Sep;42(6):763-74.
11. Gering M, Rodaway AR, Göttgens B, Patient RK, and Green AR. The SCL gene specifies haemangioblast development from early mesoderm. EMBO J. 1998 Jul 15;17(14):4029-45. DOI:10.1093/emboj/17.14.4029 |
12. Neave B, Rodaway A, Wilson SW, Patient R, and Holder N. Expression of zebrafish GATA 3 (gta3) during gastrulation and neurulation suggests a role in the specification of cell fate. Mech Dev. 1995 Jun;51(2-3):169-82. DOI:10.1016/0925-4773(95)00351-7 |
13. Casimir CM, Bu-Ghanim HN, Rodaway AR, Bentley DL, Rowe P, and Segal AW. Autosomal recessive chronic granulomatous disease caused by deletion at a dinucleotide repeat. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2753-7. DOI:10.1073/pnas.88.7.2753 |
14. Rodaway AR, Teahan CG, Casimir CM, Segal AW, and Bentley DL. Characterization of the 47-kilodalton autosomal chronic granulomatous disease protein: tissue-specific expression and transcriptional control by retinoic acid. Mol Cell Biol. 1990 Oct;10(10):5388-96. DOI:10.1128/mcb.10.10.5388-5396.1990 |
15. Rodaway AR, Sternberg MJ, and Bentley DL. Similarity in membrane proteins. Nature. 1989 Dec 7;342(6250):624. DOI:10.1038/342624a0 |

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