Turner:Solutions/GAMBITTEB
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GAMBIT TEB
| Component | Final [] | Stock [] | Units | mL stock | ' | ' |
| Tris-HCl pH 7.4 | 0.02 | 1 | M | 0.2 | ||
| NaCl | 0.2 | 5 | M | 0.4 | ||
| EDTA pH 8 | 0.001 | 0.5 | M | 0.02 | ||
| DTT | 0.002 | 0.1 | M | 0.2 | ||
| Glycerol | 10 | 50 | % | 2 | ||
| Maltose | 0.01 | 0.5 | M | 0.2 | ||
| pH to | 7.4 | with | 5N HCl | (approx) | 1 | drop |
| sterilize by | f.s. | |||||
| store at | 4C | |||||
| Note: add 1 tablet cOmplete mini, EDTA-free to 10 mL buffer before use |
