Theobaldtwang:ONE

From OpenWetWare

Jump to: navigation, search

All contents in one page!

Contents

Contact

Theobald, Tianwen Wang (an artistic interpretation)
Theobald, Tianwen Wang (an artistic interpretation)
  • Theobald Tianwen Wang
  • 130 Meilong Rd, Shanghai 200237, P.R.China
  • Email me through OpenWetWare or to address theobaldwang**gmail.com (Please replace ** with @ when you are using it.).

Education

  • Sept. 2003~June 2006, Ph.D. in Biochemical Engineering, East China University of Science and Technology
  • Sept. 2000~June 2003, M.S. in Molecular Biology and Biochemistry, Fujian Agricultural University
  • Sept. 1996~June 2000, B.S. in Microbiology, Henan Agricultural University

Training

  • Jan. 2011~Jan. 2012, Postdoc in National University of Singapore, Singapore;
2011.1-2012.1,新加坡国立大学博士后(Research fellow)
  • Jan. 2007~Nov. 2008, Postdoc in Tsinghua university, China.
2007.1-2008.11, 清华大学博士后

Employment

  • July 2006~Dec. 2006, Tianjin university;
2006年7月-2006年12月,天津大学
  • Dec.2008~Apr.2012, Jiangnan university;
2008年12月-2012年4月,江南大学
  • May 2012~present, East China University of Sci.& Tech.
2012年9月-现在,华东理工大学

Researches

  • Biochemistry
 生物化学
  • Synthetic biology
 合成生物学
  • RNA biology
 RNA生物学

Grants

  • Anti-Survivin nanobody prepared with engineered yeast and its activity in antitumor and on stem cells, funded by Municipal Supports for Pujiang Scholors of Shanghai City (Key participant)
存活素纳米抗体(Survivin-Nb)工程化酵母制备与抗肺癌及其干细胞活性研究, 上海市人才计划项目(“浦江人才”项目,F200-2R-13110,2013.09.01-2015.8.31),参与人;
  • Spatially engineering the metabolic enzymes in L-Phe biosynthesis, funded by Natural Science Foundation of China (PI);
L-苯丙氨酸的代谢酶空间组织工程研究,国家自然科学基金(青年基金,3100054,2011.1-2013.12),负责人
  • The glutathionalytion of alcohol dehydrogenase from Candida utilis under oxidative stress (PI);
氧化胁迫产朊假丝酵母醇脱氢酶的谷胱甘肽化研究(启动项目,2009-1011),负责人
  • Construction of L-phe producer strain with RED recombination and Kan/sacB selective marker (PI);
利用RED重组和Kan/sacB选择标签构建L-苯丙氨酸生产菌株,教育部高校科研基本业务费(青年探索基金,JUSRP10917,2009-2011),负责人
  • Novel technology for Large‐scale expression and purification of non-fusion recombinant protein based on the ELP-Intein system (PI).
基于ELP-Intein体系的新型非融合重组蛋白规模化表达纯化技术(教育部重点实验室开放课题,KLIB-KF201004,2011-2012),负责人

Publications

Journal articles

2013

  1. F. Ma, T.Wang, X.Ma, and P.Wang, Identification and Characterization of the Protein Encoded by ORF_382 as an L-Threonine Dehydrogenase, Protein Pept. Lett., Submitted.
  2. L.Shi, L.Tang, X.Ma, T.Wang, P. Wang, Efficient Preparation of an Anti-tumor Protein TmSm by Reversible Phase Transition of ELPs and Self-cleaving Function of Intein, China Biotechnol., 2013, 33(10):89-95.[In CHS]
  3. C.Zhang, J.Zhang, Z.Kang, G.Du, X.Yu,T.Wang,J.Chen,Enhanced production of L-phenylalanine in Corynebacterium glutamicum due to the introduction of Escherichia coli wild-type gene aroH, J. Ind. Microbiol. Biotech., 2013, 40(6):643-651. PMID:23526182. [IF2011=2.735]
  4. N. Lin, W. Zheng, L. Li, H. Liu, T. Wang, P. Wang, X. Ma*, A novel system enhancing the endosomal escapes of peptides promotes Bak BH3 peptide inducing apoptosis in lung cancer A549 cells. Targeted Oncology. 2013. DOI: 10.1007/s11523-013-0282-9, IF[2011]=3.614

2012

  1. T. Wang, A. Li, G. Du and J. Chen, A simple and universal method to express protein in its unfused form, Protein Pept. Lett., 2012, 19(9):930-933. PMID:22316309.[IF2011=1.942]
  2. T. Wang, X. Ma, G. Du and J. Chen, Overview of regulatory strategies and molecular elements in metabolic engineering, Mol. Biotechnol., 2012,52(3):300-388. PMID: 22359157.[IF2011=2.171]
  3. T. Wang, X. Ma, H.Zhu, A. Li, G. Du and J. Chen, Available methods for assembling expression cassettes for synthetic biology, Appl. Microbiol. Biotechnol., 2012, 93(5):1853-1863. PMID:22311648.[IF2011=3.425]
  4. Qiu, XF, Wang, TW, Zhong, XM, Du, GC, Chen, J , Screening and characterization of an aerobic nitrifying-denitrifying bacterium from activated sludge, Biotechnol. Bioproc. Eng., 2012, 17(2):353-360. [IF2011=1.278]
  5. Xu Y, Zheng W, Wang T, Wang P, Zhu L, Ma X, Genetic protein TmSm(T34A) enhances sensitivity of chemotherapy to breast cancer cell lines as a synergistic drug to doxorubicin, Biomed. Pharmacother., 2012, 66(5):368-372. [IF2011=2]

2011

  1. H. Zhou, X. Liao, L. Liu, T. Wang*, G. Du and J. Chen, Enhanced L-phenylalanine production by recombinant Escherichia coli BR-42 (pAP-B03) resistant to bacteriophage BP-1 via a two-stage feeding approach, J Ind. Microbiol. Biotechnol., 2011, 38(9):1219-1227. [Co-corresponding author], PMID:21104105.[IF2011=2.735]

2010

  1. T. Wang, Y. Hu, X. Liao, G. Du, J. Chen and J. Xu, Anti-oxidative stress and beyond: multiple functions of the protein glutathionylation, Protein Pept. Lett., 2010, 17(10):1234-1244. [Co-1st and -corresponding author], PMID:20394582.[IF2011=1.942]
  2. H. Zhou, X. Liao, T. Wang, G. Du and J. Chen, Enhanced l-phenylalanine biosynthesis by co-expression of pheA(fbr) and aroF(wt), Bioresour. Technol., 2010, 101(11):4151-4156. [Co-corresponding author], PMID:20137911.[IF2011=4.98]

2009

  1. T. Wang, A. Qiu, F. Meng and H. Zhou, Changing the Metal Binding Specificity of Superoxide Dismutase from Thermus thermophilus HB-27 by a Single Mutation, Mol. Biotechnol., 2009, 42(2):146-153. PMID:19191037.[IF2011=2.171]

2006

  1. T. Wang, H. Zhu, X. Ma, Z. Fei, Y. Ma and D. Wei, Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene, Appl. Microbiol. Biotechnol., 2006, 72(5):953-958. PMID:16550378.[IF2011=3.425]
  2. T. Wang, H. Zhu, X. Ma, Y. Ma and D. Wei, Structure-based stabilization of an enzyme: the case of penicillin acylase from Alcaligenes faecalis, Protein Pept. Lett., 2006, 13(2):177-183. PMID:16472081. [IF2011=1.942]
  3. T. W. Wang, H. Zhu, X. Y. Ma, T. Zhang, Y. S. Ma and D. Z. Wei, Mutant library construction in directed molecular evolution: casting a wider net, Mol. Biotechnol., 2006,34(1):55-68. PMID:16943572.[IF2011=2.171]
  4. H. Zhu, T. W. Wang, S. J. Sun, Y. L. Shen and D. Z. Wei, Chromosomal integration of the Vitreoscilla hemoglobin gene and its physiological actions in Tremella fuciformis, Appl. Microbiol. Biotechnol., 2006, 72(4):770-776. PMID:16501972.[IF2011=3.425]
  5. X. Ma, W. Zheng, T. Wang, D. Wei and Y. Ma, Optimization and high-level expression of a functional GST-tagged rHLT-B in Escherichia coli and GM1 binding ability of purified rHLT-B, J Microbiol., 2006, 44(3):293-300. PMID:16820759.[IF2011=1.095]
  6. X. Ma, W. Zheng and T. Wang, Leukocyte function-associated antigen-1: structure, function and application prospects, Protein Pept. Lett., 2006, 13(4):397-400. PMID:16712517. [IF2011=1.942]
  7. H. Zhu, R. J. Pan, T. W. Wang, Y. L. Shen and D. Z. Wei, Functional solubilization of aggregation-prone TRAIL protein facilitated by coexpressing with protein isoaspartate methyltranferase, Appl. Microbiol. Biotechnol., 2006, 72(5):1033-1038. PMID:16575568.[IF2011=3.425]
  8. X. Ma, W. Zheng, D. Wei, Y. Ma, T. Wang, J. Wang, Q. Liu and S. Yang, High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro, J Biotechnol., 2006, 123(3):367-378. PMID:16406157.[IF2011=3.045]
  9. X. Ma, W. Zheng, D. Wei, Y. Ma, T. Wang, J. Wang, Q. Liu and S. Yang, Construction, expression, and purification of HIV-TAT-survivin (T34A) mutant: a pro-apoptosis protein in Escherichia coli, Protein Expr. Purif., 2006, 47(1):36-44. PMID: 16260148.[IF2011=1.587]

2005

  1. W. Zheng, X. Ma, D. Wei, T. Wang, Y. Ma and S. Yang, Molecular cloning and bioinformatics analysis of a novel spliced variant of survivin from human breast cancer cells, DNA Seq., 2005,16(5):321-328. PMID:16329164.

Linkouts

  • Links to some labs

Protein engineering

  1. David Baker's lab: http://depts.washington.edu/bakerpg/drupal/
  2. Michael Levitt: http://csb.stanford.edu/levitt/
  3. Protein science: http://fold.doe-mbi.ucla.edu/overview
  4. Protein design lab: http://www.cmps.lu.se/biostruct/people/ingemar_andre/

Metabolic engineering and synthetic biology

  1. http://keaslinglab.lbl.gov/

SMT

  1. Xie,XS. http://bernstein.harvard.edu/pages/AboutProfXie.html

DNA chemistry

  1. http://dna.caltech.edu/DNAresearch_perspective.html
  2. https://www2.physics.ox.ac.uk/research/self-assembled-structures-and-devices
  3. http://cadnano.org/windows-installation

Enzymes

  1. http://en.wikipedia.org/wiki/Malic_enzyme
  2. http://en.wikipedia.org/wiki/Malate_dehydrogenase
  3. http://seemanlab4.chem.nyu.edu/
  • Online tools
  1. Multiple alignment: http://www.ebi.ac.uk/Tools/msa/clustalw2/
  2. Tools:http://www.nature.com/nmeth/journal/v9/n4/abs/nmeth.1923.html
  3. Signal: http://www.cbs.dtu.dk/services/SignalP/
  4. Solubility: http://mips.helmholtz-muenchen.de/prosoII/prosoII.seam#
  • Important Journals
  1. Cell: http://www.cell.com
  2. NPG: http://www.nature.com
  3. Science: http://www.sciencemag.org
  4. PNAS: http://www.pnas.org
  5. JBC: http://www.jbc.org
  6. ASM Journals: http://journlas.asm.org
  7. Biology-direct: http://www.biology-direct.com


Hottopics

De novo protein design

this article discussed some key points that should be taken into account in de novo protein design.
Personal tools