This part requires pBca1766
You have a toxic gene part that needs to be composed with pBca1766. This vector has the Pbad promoter upstream of the EcoRI site and the TrrnB terminator downstream of the BamHI site. So, you can clone your part as a basic part with the normal EcoRI/BamHI procedures and in the end get a plasmid that already has the needed regulation for our experiments. You should design your construction file to employ pBca1766-Bca1089.