Template:SBB10 ConstructionFiles JoseG sbb37

From OpenWetWare

Jump to: navigation, search

Biobricking of Pcon

PCR JG005-F and JG006-R on pBca1100-Bca1152    (66 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is Pcon-pBjk2741    {Pcon-J23100}
----------------------------
JG005-F   Cloning of papC   ccataGAATTCgtccAGATCTttgacggctagctcagtcct
JG006-R   Cloning of papC   ctgatGGATCgctagcactgtacctaggac

JCA Notes

  • This just needs to be eco/bam transferred, not pcr'd
  • Nomenclature of plasmid changed
  • Modified entry on clotho
Eco/Bam transfer pBca1100-Bca1152
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bca1152   {Pcon-J23100}
Personal tools