Template:SBB10 ConstructionFiles JoseG sbb30

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Biobricking of O99 rep Gene

PCR Pcon-F and Pcom-R on pEC52.    (1236 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is Pcon-pBjk2741    {5'UTR_repO99!}
----------------------------
JG001-F   Cloning of O99 rep Gene  ccataGAATTCgtccAGATCTaaaaacgattctgacgcatttt
JG002-R   Cloning of  O99 rep Gene   ctgatGGATCCctactctacaagacctcgtttt


JCA Notes

  • Needs to be with vector pBca9523
  • Oligo names are incorrect in description
  • Extended oligos to get to a g/c
  • Name of plasmid should be vectorName-partName (pBca9523-sbb30)
  • Corrected Clotho entry
  • Otherwise correct
Biobricking of O99 rep Gene
PCR JG001-F and JG002-R on pEC52      (1235 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144             (EcoRI/BamHI, 910+2472, L)
Product is pBca9523-sbb30    {5'UTR_repO99!}
----------------------------
JG001-F   Cloning of O99 rep Gene  
ccataGAATTCatgAGATCTaaaaacgattctgacgcattttttatg
JG002-R   Cloning of  O99 rep Gene   
CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc
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