Template:SBB10 ConstructionFiles JimH sbb11
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1) sbb11: sleeping beauty 5'TR Pool OJIMH001 through OJIMH010, assemble by PCA PCR OJIMH001/OJIMH010 on PCA reaction (257 bp, EcoRI/BamHI) Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb11 ------------ OJIMH001 PCA assembly of sbb11 CCATAGAATTCATGAGATCTAGTTGAAGTCGGAAGTTTACATACACTTAAG OJIMH002 PCA assembly of sbb11 GAAAAACGAGTTTTAATGACTCCAACTTAAGTGTATGTAAACTTCCGACTTC OJIMH003 PCA assembly of sbb11 AAGTTGGAGTCATTAAAACTCGTTTTTCAACTACACCACAAATTTCTTGTTAAC OJIMH004 PCA assembly of sbb11 CTGACTTGCCAAAACTATTGTTTGTTAACAAGAAATTTGTGGTGTAGTTGAAA OJIMH005 PCA assembly of sbb11 TTAACAAACAATAGTTTTGGCAAGTCAGTTAGGACATCTACTTTGTGC OJIMH006 PCA assembly of sbb11 ACAATTGTTGGAAAAATGACTTGTGTCATGCACAAAGTAGATGTCCTAACTG OJIMH007 PCA assembly of sbb11 GACACAAGTCATTTTTCCAACAATTGTTTACAGACAGATTATTTCACTTATAATTC OJIMH008 PCA assembly of sbb11 CCACTGGAATTGTGATACAGTGAATTATAAGTGAAATAATCTGTCTGTAAAC OJIMH009 PCA assembly of sbb11 TAATTCACTGTATCACAATTCCAGTGGGTCAGAAGTTTACATACACTAAG OJIMH010 PCA assembly of sbb11 CTGATGGATCCTTAGTGTATGTAAACTTCTGACCC
JCA Notes
- Correct
