Template:SBB10 ConstructionFiles JeniL sbb34

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Construction of Ala6 basic part sbb34
EIPCR Ala6-F/ca2741R on pBjk2741-Bca1144   (2203 bp, BglII)
Product is pBjk2741-Ala6    {<Ala6>}
----
Ala6-F  EIPCR construction of Alanine-6 part
		CCATAagatctgcagcagcagcagcagcaGGATCCtaaCTCGCTCCTCagg
jl2741R Reverse BglII oligo for Ala6 EIPCR on pBjk2741-Bca1144
		CTGAAGTGgaattcatgAGATCTATTGG

JCA Notes

  • Reverse oligo is reverse complemented
  • Should name plasmid as vectorName-partName, not vectorName-shortDescription
  • Due to similarity to other parts, reusing an oligo
  • I've also mutated out the psti for compatibility with other standards
 Construction of Ala6 basic part sbb34
 EIPCR Ala6-F/tn0002R on pBjk2741-Bca1144   (2219 bp, BglII)
 Product is pBjk2741-sbb34   {<Ala6>}
 ----
 Ala6-F  EIPCR construction of Alanine-6 part
 CCATAagatctgcTgcCgcagcagcagcaGGATCCtaaCTCGCTCCTCagg
 tn0002R	Reverse BglII oligo for Tn5 EIPCR 
 CCAATAGATCTcatgaattcCACTTCAG

Part:  gatctgcTgcCgcagcagcagcaG
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