Template:SBB10 ConstructionFiles DanielS sbb39

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PCR DS003 and DS004 on pBjh1601CK-Bjh1380  (311 bp, EcoRI/BamHI)
Sub into pBjk2741            (EcoRI/BamHI, 2170+910, L)
Product is pBjk2741-sbb39         {PmgtCB}
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DS001   Forward oligo for cloning of PmgtCB  
ccataGAATTCtccAGATCTtacggcggcaatcaggacgt
DS002   Reverse oligo for cloning of PmgtCB
gctgactataataagcgcaaattcaGGATCCatcag

JCA Notes

  • Correct, but you can do this one by eco/bam transfer
  • Need to put part into Clotho -- call it Bjh1380, not sbb39
Eco/Bam transfer pBjh1601CK-Bjh1380
Sub into pBjk2741-Bca1144            (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bjh1380   {PmgtCB}
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