Template:SBB10 ConstructionFiles CarolynK sbb32

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Construction of oriO99 Basic Part sbb32

PCR sbb32F and sbb32R on pEC52           	    (536 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144	            (EcoRI/BamHI, 2170+910, L)
Product is pBjk2741-sbb32  		    {oriO99}
-------------------------------------
sbb32F  Forward oligo for cloning of sbb32   ccataGAATTCatgAGATCTagcgcctcagcgcgccgtag
sbb32R  Reverse oligo for cloning of sbb32   ctgatGGATCCaccaaacgccaacagctcaa

JCA Notes

  • correct
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