Template:SBB10 ConstructionFiles CarolynK sbb24

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Construction of O99 5'UTR Basic Part sbb24

PCR sbb24F and sbb24R on pEC52           	    (468 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144	            (EcoRI/BamHI, 2472+918, L)
Product is pBca9523-sbb24 		    {O99 5'UTR}
-------------------------------------
sbb24F  Forward oligo for cloning of sbb24  ccataGAATTCatgAGATCTacgattctgacgcattttttatg
sbb24R  Reverse oligo for cloning of sbb24  ctgatGGATCCacgtcaaaaaccagccagaactgcg

JCA Notes

  • Vector sizes slightly off, but otherwise correct
 PCR sbb24F and sbb24R on pEC52           	    (468 bp, EcoRI/BamHI)
 Sub into pBca9523-Bca1144	            (EcoRI/BamHI, 2472+910, L)
 Product is pBca9523-sbb24 		    {O99 5'UTR}
 -------------------------------------
 sbb24F  Forward oligo for cloning of sbb24  ccataGAATTCatgAGATCTacgattctgacgcattttttatg
 sbb24R  Reverse oligo for cloning of sbb24  ctgatGGATCCacgtcaaaaaccagccagaactgcg
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