Template:SBB09 38352

From OpenWetWare

Jump to: navigation, search

Eric Hsieh

Welcome to your project page!

For each part listed, you should:

  • Design oligos to make your part
  • Write up proper construction files and put it on the Construction Files page
  • Put your oligos on the Oligo Log page
  • Put your part sequence on the part document
  • Name your parts according to the list here

Ice Nucleation Repeats

 Source: pBca1256-Bca1346

Ice nucleation protein is one of our display systems, but it has an interesting feature that may be useful for introducing in other display systems. It has many repeats of:

              [.........48.residues.repeated.domain..........]
             /              / |              | \              \
            AGYGSTxTagxxssli  AGYGSTxTagxxsxlt  AGYGSTxTaqxxsxlt
            [16.residues...]  [16.residues...]  [16.residues...]

From http://www.ebi.ac.uk/interpro/IEntry?ac=IPR000258

You can download the template map for your part here. You will be making a BglBrick passenger part derived from the navy blue region.

Here is a starter reference for background: PMID: 16233340

This part encodes a passenger protein

Your passenger part should be of the {<part>} style (no start, no stop). There should be NO prepro sequence in your part. If your part is naturally secreted, you should encode only the active peptide. You should design your construction file to insert your part into plasmid pBca9495AK-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.


Leucine Zipper Peptide IILK

 Source: pBca1256-mm011  {<GSGS>}{<GSGS>}{<pCAP-IILK>}

This part has already been constructed as a BglBricks basic part in plasmid pBca1256. You will be transferring the part using the Gateway reaction into the assembly vector. To do this, you will react the source plasmid with plasmid pBca1254AK. As background, you should read PMID: 12459719.

This part encodes a passenger protein

After performing the Gateway reaction, your product plasmid will be pBca9495AK-partname. To see what the part looks like in the product vector, look at this map of the RFP-expressing part Bca1144 in pBca9495AK.

Personal tools