Template:SBB09 19607

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Roger Lowe

Welcome to your project page!

For each part listed, you should:

  • Design oligos to make your part
  • Write up proper construction files and put it on the Construction Files page
  • Put your oligos on the Oligo Log page
  • Put your part sequence on the part document
  • Name your parts according to the list here

Intimin (refactored)

 Source:  E. coli strain O157:H7

For background, you should read PMID: 11717287. You will be making a part derived from the eaeA gene which encodes the Intimin protein. You can download an annotated sequence here. You will be making a part out of just the pink regions of this file. Do not include the green prepro region.

Note: You will be sharing the reverse oligo with whoever gets the SBB09_18094 project. You should find this person and design that oligo together.

This part encodes a C-terminal display protein

Your displayer part should be of the {<part>} style (no start, no stop). The source sequence for your part likely contains a prepro sequence. You should omit that region of the sequence in designing your part.

You should design your construction file to insert your part into plasmid pBca9495CA-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.


Aluminum-Binding Peptide

 Source: synthetic

You should read PMID: 15703906. You will make a BglBrick encoding:

 VPSSGPQDTRTT

No start, no stop, in frame.

This part encodes a passenger protein

Your passenger part should be of the {<part>} style (no start, no stop). There should be NO prepro sequence in your part. If your part is naturally secreted, you should encode only the active peptide. You should design your construction file to insert your part into plasmid pBca9495AK-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.

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