First, We constructed shrunk DNA origami and the result is shown in Fig. 12 (a). As you can see, shrunk DNA origami was successfully constructed. Most Origamis are shrunk by 9 nm as compared to original DNA origami (Fig. 12 (b)) by blunt-end π–π stacking interactions within a molecule.
Fig. 12 (a). Schematic image of Diagonal shrunk DNA origami.
Fig. 12 (b). Schematic image of Zig-Zag shrunk DNA origami.
Fig. 12 (c). Schematic image of original DNA origami.
Cyclops and Smiley DNA origami
Second, we prepared cyclops DNA Origami. Cyclops DNA Origami is not using staple strands in middle of Smiley DNA origami. We shown Fig. 13 to the result of AFM observation. This result show that the one-eyed smiley “Cyclops” was prepared. As expected, smiley DNA Origami was shrunk by blunt-end π–π stacking interactions within a molecule. Farther, we added staple strands of middle of Smiley DNA origami. In the result, cyclops DNA origami transformed the smiley DNA origami (Fig. 14).
Fig. 13. Schematic image of Cyclops DNA origami.
Fig. 14. Schematic image of Cyclops to Smiley DNA origami.
Bear Trap DNA origami
Third, we prepared DNA origami having a capability of shape selective capturing “Bear Trap”. We shown Fig.15 to the result of AFM observation. This result suggest that the architecture was formed Origami with the square hole in the center by π- π stacking interaction. We prepared plus shaped DNA origami for allow it to Bear Trap. The result showed string of plus shaped Origami. The next time, We mixed Bear Trap with plus shaped DNA origami. Against the odds, plus shaped DNA origami was not captured Bear Trap. Because we thought that Bear Trap's π- π stacking interaction is too strong.
Fig. 15. Schematic image of Bear Trap DNA origami.
Fig. 16. Schematic image of Plus shaped DNA origami.
Fig. 17. Schematic image of Bear Trap and Plus shaped DNA origami mixture.