Talk:Standard E. coli Strain for BioBricks

From OpenWetWare

Jump to: navigation, search

A paper that I ran across - "Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway.", by Flores et al. Short version - they overexpressed G6PDH and largely restored the growth rate of a strain carrying a high copy plasmid. Doesn't solve the problem of protein load, but it might be useful in a BB strain, particularly for constructing (non-functional or non-induced) intermediates. -- Josh

I have a folder with all the work that Bram and Caitlin did on building the KanR/PheS expression cassette, which I can pass on to anyone who wants to use it in the short term --BC 16:36, 8 Jun 2005 (EDT)

Can this information be loaded into the wiki easily? --Reshma 20:00, 8 Jun 2005 (EDT)

From F. R. Blattner et al. The Complete Genome Sequence of Escherichia coli K-12 Science, 277(5331):1453-1462, 1997

We chose strain MG1655 as the representative to sequence because it has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange, respectively. --Jgritton 11:30, 11 Jul 2005 (EDT)
Personal tools