Talk:IGEM:MIT/2006/Notebook/2006-6-6

Enzyme information template

1. Species
2. Sequence & Structure
   • Codon optimized?
   • Restriction sites
   • Post-translational modification
   • Material availability
     • cDNA from arabadopsis
     • Request from authors
       • Form of DNA?
     • need to synthesize?
3. Reaction catalyzed
   • Substrate
     • Synthesized in our chassis or supplied exogenously?
   • Product
     • Coolness (Austin)
   • Reaction rates - K_m,k_cat
4. Does it smell??
   • Regulation issues
   • Transport issues for substrate/product
5. Ported to E. coli or yeast?
6. What is the regulation in planta
7. References & Links
   • Corresponding authors
   • Genbank accession numbers
   • Pubmed
   • Websites
   • Any companies or patents?