Talk:CH391L/S12/Origins of Replication
- Jeffrey E. Barrick 12:19, 7 March 2012 (EST):What is the origin on the iGEM plasmid that BioBricks are distributed in?
The section "Plasmid backbones for advanced users" has almost nothing in it, except for a brief mention of pMB1/ColE1.
The section "Plasmid backbones that comply with other assembly standards" has a link called Other assembly standard plasmid backbones that list a few labs. The are four labs that meet the standards for a backbone, but only one of them (Berkeley) lists their oris (They use pSC101 and RK2/oriV).
"Miscellaneous other plasmid backbones" mentions pMB1/ColE1, P15A, F, and R6K.
If I were an iGEMer and wanted to build constructs, I would use a pMB1/ColE1 backbone (e.g. pUC, pET, pBR322 etc). Their higher copy allows for easier cloning and sequencing. P15A backbones (e.g. pACYC, pBad) are next best choice, if you need a two-plasmid system, but their copy numbers are lower, so they are kind of a pain for routine cloning.
- Jeffrey E. Barrick 12:19, 7 March 2012 (EST):What is the highest known copy number of any plasmid?
- Adam Meyer 10:37, 8 March 2012 (EST):The highest one I am aware of pTZ, which is a derivative of pMB1. It is supposed to have a copy number over 1000.