Talk:CH391L/S12/Origins of Replication

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  • Jeffrey E. Barrick 12:19, 7 March 2012 (EST):What is the origin on the iGEM plasmid that BioBricks are distributed in?

The section "Commonly used BioBrick plasmid backbones" has links to Assembly and Operation. These are very similar and suggest pMB1/ColE1, pSC101, P15A, F, and P1.

The section "Plasmid backbones for advanced users" has almost nothing in it, except for a brief mention of pMB1/ColE1.

The section "Plasmid backbones that comply with other assembly standards" has a link called Other assembly standard plasmid backbones that list a few labs. The are four labs that meet the standards for a backbone, but only one of them (Berkeley) lists their oris (They use pSC101 and RK2/oriV).

"Miscellaneous other plasmid backbones" mentions pMB1/ColE1, P15A, F, and R6K.

If I were an iGEMer and wanted to build constructs, I would use a pMB1/ColE1 backbone (e.g. pUC, pET, pBR322 etc). Their higher copy allows for easier cloning and sequencing. P15A backbones (e.g. pACYC, pBad) are next best choice, if you need a two-plasmid system, but their copy numbers are lower, so they are kind of a pain for routine cloning.

  • Jeffrey E. Barrick 12:19, 7 March 2012 (EST):What is the highest known copy number of any plasmid?
    • Adam Meyer 10:37, 8 March 2012 (EST):The highest one I am aware of pTZ, which is a derivative of pMB1. It is supposed to have a copy number over 1000.
  • --Erik Quandt 10:29, 11 March 2012 (EDT): I have seen the plasmid pBBR1 mentioned quite a bit in regards to its broad host range. Did you come across it in your research? Anybody have it?
    • Adam Meyer 23:04, 11 March 2012 (EDT):I hadn't come across, but I did find it on addgene, plasmid # 14703
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