Talk:20.109(S12):Transcript-level analysis (Day6)

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Contents

qPCR data

T/R

The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (The latter is likely to be more reliable.)

Media:20109-S12_TR_qPCR.txt

W/F

The text file below contains all of the W/RF data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (The latter is likely to be more reliable.)

Media:20109-S12_WF_qPCR.txt

And here is a new set of data when point C3 (rises very quickly) is excluded when determining the "all" and "CN II" noisebands.

Media:20109-S12_WF_qPCR-corrected.txt

qPCR section-wide curves

T/R section, Raw amplification curves
T/R section, Raw amplification curves
T/R section, Log-scale amplification curves with noise threshold
T/R section, Log-scale amplification curves with noise threshold
T/R section, Log-scale 18S curves
T/R section, Log-scale 18S curves
T/R section, Log-scale CN II curves
T/R section, Log-scale CN II curves
T/R section, Log-scale CN I curves
T/R section, Log-scale CN I curves
T/R section, Melt curves for 18S samples
T/R section, Melt curves for 18S samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples


W/F section, Raw amplification curves
W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Log-scale amplification curves with noise threshold
W/F section, Log-scale 18S curves
W/F section, Log-scale 18S curves
W/F section, Log-scale CN II curves
W/F section, Log-scale CN II curves
W/F section, Log-scale CN I curves
W/F section, Log-scale CN I curves
W/F section, Melt curves for 18S samples
W/F section, Melt curves for 18S samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN I samples
W/F section, Melt curves for CN I samples
W/F section, Log-scale amplification curves with noise threshold, well C3 excluded
W/F section, Log-scale amplification curves with noise threshold, well C3 excluded
W/F section, Log-scale CN II curves, well C3 excluded
W/F section, Log-scale CN II curves, well C3 excluded


Cross-group meetings

Scheme

Red 1/Pink 1

Red 2/Purple 1

Orange 1/Green 2

Orange 2/Blue 2

Yellow 2/Blue 1

Green 1/Purple 2

Pink 2/Yellow 1

T/R

Taleen/Ruisi

Jessica/Kristen

Nathan/Shambhu

Jeff/Fangdi from W/F section if possible

Angel/Michael

Esme/Cher

Maddy/Meena

W/F

Dorian/Lauren

Sarah/Dharshan

Sixing/Carlos

Samira/Nina

Melina/Isabella

Brian/Matt

Holly/Rachel

Fangdi/Jeff from T/R section if possible

(Brian and Carlos I often mix-up which one of you sits where, but please stick with the above for simplicity. Thanks!)

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