Talk:20.109(S12):Induce protein and evaluate DNA (Day5)

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Contents

Induced pellet example

Examples of induced (left) and control (right) cell pellets
Examples of induced (left) and control (right) cell pellets


TR O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD M124S/E67K/T79P OD Chosen mutant Pellets yellow?
Red
Orange .067 .024 Mutant 1: .095 / Mutant 2: .065 Yes
Yellow 0.069 0.138 0.062 Yes
Green 0.062 0.150 0.140 Yes
Blue
Pink 0.071 0.317 0.119 Y: bright yellow green WT, F65D; dull/pale yellow T79P
Purple 0.0771 0.1352 0.0464 Yes

Gels

T/R Orange
T/R Orange
T/R Yellow
T/R Yellow
T/R Green
T/R Green
T/R Blue NOTE: Gel Was Run Backwards
T/R Blue NOTE: Gel Was Run Backwards
T/R Pink
T/R Pink



WF O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD M124S/E67K/T79P OD Chosen mutant Pellets yellow?
Red .247 .047 .05 Yes
Orange 0.057 0.273 0.381 The pellets were yellow.
Yellow 0.387 (actually T79P) 0.118 (E67K) 0.095 Y
Green 0.396 0.105 0.094 Yes
Blue 0.366 (actually T79P) 0.109 (E67K) 0.102 Y
Pink 0.242 (actually T79P) 0.089 (E67K) 0.066 Yes
Purple 0.263 (actually T79P) 0.300 (M124S) 0.063 Yes

Gels

W/F Red
W/F Red
W/F Orange
W/F Orange
W/F Yellow
W/F Yellow
W/F Green
W/F Green
W/F Blue -- NOTE: Lanes 8 and 9 are switched
W/F Blue -- NOTE: Lanes 8 and 9 are switched
W/F Pink
W/F Pink
W/F Purple
W/F Purple



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