Talk:20.109(F11): Mod 1 Day 7 FACS analysis

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Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

T/R FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.
team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) mock data EGFP data delta 5 data delta 3 data delta5+delta3 delta5+delta3(Pme) delta5+delta3(Bam) extra experiment (specify below)
Red I = 12:30-2PM 0.00 50.51 0.00 0.00 1.91 4.98 3.89 not done
Orange III = 2:30-4PM 0.00 35.48 0.00 0.01 1.11 1.98 1.86 not done
Yellow I = 12:30-2PM 0.00 45.28 0.00 0.00 0.89 3.33 2.59 Not done
Green I = 12:30-2PM 0.00 58.5 0.01 0.01 1.61 4.33 4.92 ethidium bromide (100 ng/ml), 0.24
Blue II = 1:30-3PM 0.00 54.41 0.00 0.00 1.38 4.01 3.54 Not done
Pink II = 1:30-3PM 0.00 46.04 0.01 0.00 1.19 3.97 2.69 Not done
Purple II = 1:30-3PM 0.00 18.14 0.00 0.02 1.32 0.49 1.06 Not done

W/F FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.
team color FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) mock data EGFP data delta 5 data delta 3 data delta5+delta3 delta5+delta3(Pme) delta5+delta3(Bam) extra experiment (specify below)
Red III = 2:30-4PM 0.00 64.35 0.01 0.02 0.01 0.02 0.02 not done
Orange II = 1:30-3PM 0.00 wasn't saved :( 0.02 0.00 1.02 4.74 0.00 not done
Yellow I = 12:30-2PM 0.00 56.33 0.00 0.00 2.14 6.34 6.50 0.89 (2:1 Δ5:Δ3, 0.15 μg DNA)
Green II = 1:30-3PM 0.00 48.05 0.01 0.00 2.25 5.47 6.54 4.34 (50:50 of each Δ3 cut type added, 0.20 μg DNA total)
Blue I = 12:30-2PM 0.00 17.99 0.00 0.00 1.36 8.55 6.00 1.40 (3:1 Δ5:Δ3, 0.20 μg DNA total)
Pink III = 2:30-4PM 0.00 52.67 0.01 0.00 1.46 4.39 3.80 not done
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