Talk:20.109(F09): Mod 1 Day 6 Restriction map and tissue culture

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Part 1: Agarose Gel Electrophoresis

Your samples were run on a 1% agarose gel at 125 volts for 30-45 min. This is the order samples should have been loaded. If you did yours differently, please note under the image of your gel.

Lane Plasmid sample volume to load
1 pCX-NNX uncut 20 μL
2 cut with enzyme 1 20 μL
3 cut with enzyme 2 20 μL
4 cut with enzymes 1 and 2 20 μL
5 1 kb marker 10 μL
6 candidate 1 uncut 20 μL
7 cut with enzyme 1 20 μL
8 cut with enzyme 2 20 μL
9 cut with enzymes 1 and 2 20 μL
10 candidate 2 uncut 20 μL
11 cut with enzyme 1 20 μL
12 cut with enzyme 2 20 μL
13 cut with enzymes 1 and 2 20 μL
14 1 kb marker 10 μL

T/R lab

Below is an image of your gel of the diagnostic digest. Please fill in the enzymes you used and what bands you expected for your double digest

Red Orange Yellow Green
Enzymes: BamHI & XhoI

Expected double digest bands: 1180 and 4158

Enzymes: BamHI & XhoI

Expected double digest bands: 1180 and 4160

Enzymes: BamHI, SalI

Expected double digest bands: 3800, 1600

Enzymes: XhoI, EcoRV

Expected double digest bands: 547bp, ~4797bp

Blue Purple Pink
Enzymes: BamHI & Xho1

Expected double digest bands: 1172bp & 4170 bp

Enzymes: BamHI & XhoI

Expected double digest bands: 1184bp & 4160bp

Enzymes: BamHI & Xho1

Expected double digest bands: 1198bp & 4160bp

W/F lab

Below is an image of your gel of the diagnostic digest. Please fill in what enzymes you used and the bands you expected for your double digest

Red Green Blue Purple
Enzymes: Xho1 and BamH1

Expected double digest bands: 4160 and 1220

Enzymes: BamH1 and xho1

Expected double digest bands: 1629 and 3701

Enzymes: BamHI and NotI

Expected double digest bands: 1214 and 4132

Enzymes: BamHI and Xho1

Expected double digest bands: 1180 and 4160

Pink
Enzymes: BamH1 XhoI

Expected double digest bands: 1629 and 3701

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