Talk:20.109(F08): Mod 2 Day 2 Yeast transformation

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Agarose Gel of PCR Products from Mod 2, Day 1

Tuesday/Thursday lab (run on 10/9/08)

Image:081009_TR_PCRGel.BMP

Lanes

1: 1 kb ladder (Datasheet)

2: Red team, + template

3: Red team, - template

4: Orange team, + template (SEE NOTE BELOW)

5: Orange team, - template

6: Yellow team, + template (SEE NOTE BELOW)

7: Yellow team, - template

8: Green team, + template

9: Green team, - template

10: Pink team, + template

11: Pink team, - template

12: Purple team, + template

13: Purple team, - template

14: 1 kb ladder (Datasheet)

Wednesday/Friday lab (run on 10/10/08)

Image:081010_PCR_WF.BMP

Lanes

1: 1 kb ladder (Datasheet)

2: Blue team (T/R lab), + template

3: Blue team (T/R lab), - template

4: Yellow team, + template

5: Yellow team, - template

6: Green team, + template

7: Green team, - template

8: Blue team, + template

9: Blue team, - template

10: Pink team, + template (SEE NOTE BELOW)

11: Pink team, - template

12: Purple team, + template

13: Purple team, - template

14: 1 kb ladder (Datasheet)

NOTE:

Some of the samples may have been mixed with a bad batch of loading dye or there was too much ethanol remaining in the sample from the clean-up kit or some other unknown. Whatever the reason, the samples floated out of the well of the gel, instead of sinking to the bottom of the well. This caused the samples to be lost and they cannot be visualized well on the gel. Thus, we cannot check if PCR product was produced for some of the teams. Luckily, a tiny bit of the sample from Yellow Team (T/R lab) could be run, and a small band can be visualized, confirming the PCR worked. As this was a diagnostic test, it would have been nice if it worked, but it is not essential to continue on with the rest of the lab.

---Brian

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