Talk:20.109(S12):Purify RNA and run affinity column (Day4)
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T/R
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Notes (e.g., short justification for work, whether any other parameter was changed, etc.) | Note changes to plan made on D4 (if any) here |
|---|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 34 | Trying maximum wash number done in past. | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | 2% | 4 | 24 | following same protocol from Spring 2010 with the intention of comparing results. | |
| 2 | Orange | 1 | 4 | 24 | To examine the enrichment at a lower concentration of 8-12. | |
| 3 | Yellow | 0.25 | 4 | 24 | To get a sense for the level of enrichment at a low concentration and a high number of washes | |
| 4 | Green | 1 | 8 | 36 | comparing a number of washes (8) that provides decent result with 36 to find maximum number of washes that provides a decent yield | |
| 5 | Blue | 10 | 10 | 30 | 30 is around max number done in the past. | none yet |
| 6 | Pink | 0.5 | 4 | 24 | Trying a lower 8-12% (only 0.5) because it failed last year | |
| 7 | Purple | 10% | 4 | 24 | we want to repeat this experiment exactly as it was done in the past. |
W/F
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
| Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Notes (e.g., short justification for work, whether any other parameter was changed, etc.) | Note changes to plan made on D4 (if any) here |
|---|---|---|---|---|---|---|
| 0 | Sample | 5 | 5 | 34 | Trying maximum wash number done in past. | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
| 1 | Red | 10 | 14 | 20 | Exploring the minimum wash number necessary to have sufficient enrichment. However, also interested in potentially doing 3 data points (if possible)! | |
| 2 | Orange | 0.5 | 4 | 24 | Examine how very low concentration of '8-12' can effect enrichment. | |
| 3 | Yellow | 1 | 4 | 24 | Examine enrichment using a low concentration of 8-12. | |
| 4 | Green | 50 | 4 | 24 | Spring 2011 repeat | |
| 5 | Blue | 10 | 8 | 16 | Spring 2010 repeat | |
| 6 | Pink | 10 | 2 | 34 | Repeating from S11 | |
| 7 | Purple | 2 | 20 | 20 | Exploring effects of different temperatures (25C, 4C) |
