Talk:20.109(S11):Prepare DNA for cloning (Day3)
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T/R
Record of purification gel
Control digest gel
If you digested for longer than 1 hr or otherwise changed the protocol, please add that to the "Notes" section in the table below.
Lane # | Group | Sample | Notes |
---|---|---|---|
1 | Red | XmaI | |
2 | Orange | BamHI | |
3 | Orange | BamHI | |
4 | Yellow | XmaI | |
5 | N/A | Undigested | 2.5 μL DNA |
6 | N/A | 10 μL Ladder | 1 Kbp size from NEB |
7 | Green | BamHI | |
8 | Blue | XmaI | |
9 | Pink | BamHI | |
10 | Purple | XmaI |
W/F
Record of purification gel
Control digest gel
If you digested for longer than 1 hr or otherwise changed the protocol, please add that to the "Notes" section in the table below.
Lane # | Group | Sample | Notes |
---|---|---|---|
1 | N/A | 10 μL Ladder | 1 Kbp size from NEB |
2 | Red | XmaI | |
3 | Orange | BamHI | |
4 | Yellow | XmaI | |
5 | N/A | Undigested | 2.5 μL DNA |
6 | Green | BamHI | |
7 | Blue | XmaI | |
8 | Pink | BamHI | |
9 | Purple | XmaI | |
10 | N/A | 10 μL Ladder | 1 Kbp size from NEB |