Talk:20.109(S08):Induce protein expression (Day4)

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Examples of induced (left) and control (right) cell pellets
Examples of induced (left) and control (right) cell pellets


TR O/N cultures

The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.

Group red: the -IPTG value for your W2 sample was 0.0537.

Group OD WT OD Mut1 OD Mut2 Pellets yellow?
Green 0.118 0.070 D1 0.072 H1 Y, but D1 weak
Purple 0.123 0.087 K1 0.085 E2 Y
Blue 0.104 0.111 D2 0.047 P2 Y
Red 0.131 0.085 R2 0.068 W2 Y

WF O/N cultures

The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.

Group OD WT OD Mut1 OD Mut2 Pellets yellow?
Green 0.063 0.087 H2 0.076 A2 Y
Purple 0.073 0.093 L1 0.088 T2 Y
Yellow 0.075 0.077 K1 0.077 E3 Y
Red 0.078 0.084 P2 0.080 W1 Y
Pink 0.072 0.310 P1 0.067 R2 WT-Y, Mut-N or v. weak
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