Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your assignments if you so choose.

T/R lab

Red	Orange	Yellow	Green
Blue	Purple	Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

Row 1 legend:

1. Red backbone
2. Red fragment
3. 1 kb ladder
4. Orange backbone
5. Orange fragment
6. Yellow backbone
7. Yellow fragment
8. 1 kb ladder
9. Green backbone
10. Green fragment

Row 2 legend:

1. Blue backbone
2. Blue fragment
3. 1 kb ladder
4. Pink backbone
5. Pink fragment
6. 1 kb ladder
7. Purple backbone

W/F Lab

Red	Orange	Yellow	Green
Blue	Purple	Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

WF Legend:

1. Green backbone
2. Green fragment
3. 1 kb ladder
4. Blue backbone
5. Blue fragment
6. Pink backbone
7. Pink fragment
8. 1 kb ladder
9. Purple backbone
10. Purple fragment

You can upload the pre-purification gels for reference here

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!

Green Group

Blue Group

Error creating thumbnail: File with dimensions greater than 12.5 MP

Pink Group

Purple Group

PINK Group