Synthetic complete (SC) medium

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Dropout mix

  • A dry powder mix of all amino acids and nucleic acids.
  • Omit the components used for selection
  • Mix end over end with clean glass marbles 15 minutes.


  • Adenine 0.5 g
  • Alanine 2.0 g
  • Arginine 2.0 g
  • Asparagine 2.0 g
  • Aspartic acid 2.0 g
  • Cysteine 2.0 g
  • Glutamine 2.0 g
  • Glutamic acid 2.0 g
  • Glycine 2.0 g
  • Histidine 2.0 g
  • Inositol 2.0 g
  • Isoleucine 2.0 g
  • Leucine 10.0 g
  • Lysine 2.0 g
  • Methionine 2.0 g
  • p-aminobenzoic acid 2.0 g
  • Phenylalanine 2.0 g
  • Proline 2.0 g
  • Serine 2.0 g
  • Threonine 2.0 g
  • Tryptophan 2.0 g
  • Tyrosine 2.0 g
  • Uracil 2.0 g
  • Valine 2.0 g

Total weight 54 g


For SC -ura -trp dropout medium additions to SORB plates

  • Total weight of mix is 50 g
  • For SORB plates with 0.6 g/liter SC dropout medium addition
    • Add 24 mg/liter of Uracil or 24 ml/liter of an 0.1% solution
    • Add 24 mg/liter of Tryptophan or 2.4 ml/liter of a 1% solution

Final SC medium, per liter, autoclave

  • Bacto-yeast nitrogen base w/o amino acids (0.67%) 6.7 g
  • Glucose (2%) 20 g
  • Dropout mix 2.0 g
  • Bacto Agar (2%) 20 g
  • Add 80 mg/liter of Uracil or 80 ml/liter of an 0.1% solution
  • Add 80 mg/liter of Tryptophan or 8 ml/liter of a 1% solution
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