SynBioBootcamp:Notebook/Shane Peralta
From OpenWetWare
(Redirected from SynBioBootcamp:Notebook/BioE 140L Spring 2011 Lab)
Entry 1: 10 February 2011
Hai gais, amidoinitrite?
Entry 2: 15 February 2011
Today I...
- Set up all my oligos/templates/buffers in my pcr tubes. I pretty much just followed the first few parts of the PCR cloning protocol, to the letter.
Entry 3: 17 February 2011
Today I...
- Ran my PCR products on an analytical gel (you know, just to check). Everything was as expected.
- Did a regular Zymo cleanup on my products. I fear that I may have mislabeled one of my tubes since I had 2 that said sbb1113 and none that said sbb1119. I just changed one to 1119. Let's hope for the best.
Entry 4: 22 February 2011
It looks like someone gave us the wrong buffer or something, according to the prof. I guess he redid everyones products. Lucky!
Anyway, today I...
- Digested my products with EcoRI and BamHI, according to the digest protocol.
- Ran a gel and then cut my products out of it. That was pretty interesting, but I think I spent too much time in the UV because my eyes kinda hurt.
Entry 5: 24 February 2011
Today I...
- Ran a Zymo gel purification, according to protocol.
Entry 6: 1 March 2011
Today I...
- Ligated my digests into plasmids.
- Transformed bacteria with the plasmids. Yay! It's like I've got 3 plates full of bacterial pets. I hope they grow up big and strong.
Entry 7: 3 March 2011
Today I...
- Did a miniprep. Originally I had 12 good colonies (4 vials for each part), but 2 of my 1113s turned pink after I pelleted. Too bad. Also, I had to kill all the bacteria that grew in the vials. So sad. Oh well, they gave up their lives for a greater purpose: my project.
Entry 8: 8 March 2011
I showed up to class an hour-and-a-half late. Stupid alarm.
Anyway, today I...
