Springer Lab:Microscopes

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Contents

Nikon Ti microscope)

Sign-up list

User / Date / Duration

Configuration

General

Objectives

Coming soon...

Cameras

Filter cubes

  • 1. DAPI (Semrock LF405-A: excitation 390/40, dichroic 405, emission 452/45)
  • 2. CFP (excitation 438/24, dichroic 458, emission 483/32)
  • 3. GFP (excitation 472/30, dichroic 495, emission 520/35)
  • 4. YFP (excitation 500/24, dichroic 520, emission 542/27)
  • 5. RFP (excitation 562/40, dichroic 593, emission 641/75), this filter was designed for true RFPs like mCherry
  • 6. Bf/Phase



filters for the lasers

Coming soon....

Lasers

Maintenance log

  • Install new DAPI filter set. A new cfg file was genereated for micromanager.
  • 2011.05.06 Install new versions of micromanager 1.3.46, 1.3.41
  • 2011.05.06 Changed drivers versions from

Ti Control: Ver 1.0.54
Ti SDK: Ver 1.0.78
Eclipse Ti: Ver.V.2.10


To-do list:

  • Matlab problems with PSF. Cannot detect the state of the PSF.
  • The PSF does not engage when moving in several positions of the plate.



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