Spectrophotometer, Dual Beam
Shimadzu Dual Beam Spectrophotometer
1. New user MUST be trained by lab RA or present users
HOW TO OPERATE:
The Shimadzu spectophotometer is a dual beam instrument capable of doing thermal melts
and is particularly useful for kinetic experiments since it is dual beam. However, there is no thermal control for regular cells.
For thermal melt analysis it utilizes a special 8 channel cell that is on microtitre plate spacing.
The spec can be used without the computer as a stand alone instrument and data taken off via USB drive. The computer is better for more complicated experiments and is required for thermal melts. The computer is
not networked and does not have a printer, transfer data with USB drive. I'll train one person per lab, some reading may be required.
PDF files with instructions and paper manuals are on/near the machine.
Micro and submicro cells require a Z height of 15mm
Suitable cells for micro volume work are:
16.50-Q-10/Z15, 50ul volume self masking with 10mm path length
16.100-Q-10/Z15, 100ul volume self masking with 10mm path length
The cell for thermal melts is:
Cat # 660-236-40, Type 660.236-QS
See Document: Image:UV-1800 Operation Manuyal.pdf
See Document: Image:UV-1800 System Manual.pdf
See Document: Image:Shimadzu spec notes.pdf
--Jessica Metzger 17:52, 17 December 2012 (EST)