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than the running buffer (so that the samples sink in the well). Ficoll sucrose glycerol *sieving agarose The precise amount of dye is not important. However...

Buffer (0.5M Sucrose, 10% glycerol). 7. Centrifuge cells for 15 mins at 3000g. 8. Resuspend pellet in 3ml Electroporation Buffer (0.5M Sucrose, 10% glycerol)...

MRS media Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6) Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino...

0.5M Sucrose, 0.1M MgCl2 Species = L. fermentum Dilution = 1/50 Growth = 1% Glycine, 37°C Wash = 5mM NaH2PO4 1mM MgCl2 (0°C) Buffer = 0.9M Sucrose 3mM MgCl2...

solution of MgCl2 Stock Solution of Glucose Electroporation Buffer (0.5M Sucrose, 10% Glycerol) Grow cells overnight in MRS Broth at 30°C. Use overnight...

paraformaldehyde (100 mls PBS) 3 g paraformaldehyde 2 g sucrose 0.5% Triton X-100 20 mM Tris pH 7.4 50 mM NaCl 300 mM sucrose 3 mM MgCl2 BSA 10mg/ml in PBS 0.05% NP-40...

a few months. Buffer E 10mM Tris (pH 7.5), 2mM MgCl2, 270mM sucrose. Combine 46.2g sucrose, 5mL 1M Tris (pH 7.5), and 1mL 1M MgCl2, adjust volume to 500mL...

solution 1, 3x wash volume so ~30ml R1: Tris-HCl + 0.5M sucrose, 30ml EDTA, 0.1M, 3.3ml SMM = sucrose, 0.5M , , 30 + 3x wash volume, so ~60ml W2: SMM for washing...

Ficoll™ is a sucrose polymer commonly used to adjust the density and viscosity of solutions in the lab. It is employed in density gradients and loading...

H1138 horse serum, donor herd, heat inactivated, 500 ml 2x EMD OmniPur Sucrose 500g 3mm glass balls (for spreading transformed cells on plates)- no more...

Heart Infusion Broth (Difco 0038) 17.5 g Sucrose 40 g Agar Noble (Difco 0142) if necessary 12 g DI Water 710 ml...

sugar, i.e. sucrose). HONING IN ON THE ASPECT OF CONTROL: Try to regulate the system with sucrose (or dextran**) so that the presence of sucrose (or dextran...

gradient of 15% sucrose until tissue sinks, then 25% sucrose until tissue sinks, then 30% sucrose until tissue sinks. Sucrose cryopreservative (antifreeze)...

allows for downhill transport of arabinose, as well as maltose, palatinose, sucrose, and cellobiose (3), but does not actively transport these sugars (4). Lactose...

ice-cold 0.5M Sucrose, 10% glycerol. Repeat. Concentrate cells: Centrifuge culture for 5min at 4000g Resuspend in 0.8ml ice-cold 0.5M Sucrose, 10% glycerol...

(MOI = 0.01, ~3 days postinfection) 1X PBS, 4°C 10 mM TRIS.CL, pH 9.0 36% sucrose in 10 mM TRIS.CL, pH 9.0 1 mM TRIS.CL, pH 9.0 Equipment Sterile rubber policeman...

a) How many grams of sucrose (MW=342) would you need to make 100mL of a 10-2 M sucrose solution? b) How many milligrams of sucrose would you need to make...

Cryoprotection: Cryoprotected in 30% Sucrose in 0.1M PBS Material label and storage: Labeled as RC-001 and stored in sucrose in a vial prior to being cut. The...

leaving the embryo in 0.1M PB 30% Sucrose solution overnight. Material label and storage: Labeled as MH001 and stored in a sucrose filled vial. Objective: Practiced...

almost 3 hour. Prepare 50 ml of 200 mM sucrose solution and 50 ml of 200 mM glucose solution. Add 1 ml of sucrose solution in each electroformation cell...