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  • Endy:Screening plasmid/Inverter characterization (section Measured with older versions of screening plasmid (I13537.pSB1A2, I13538.pSB1A2, or I13534.pSB4A3))
    transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. DOI:10...
    4 KB (528 words) - 20:38, 26 July 2006
  • Synthetic Biology:Vectors/Inventory (section pSB1A2-P1010)
    Tom Knight (formerly known as pSB1A2-1) derived from pSB1A1 constructed by Leon Chan (formerly known as pSB1A3-1 or pSB104.2) purpose: base plasmid used...
    5 KB (626 words) - 12:14, 25 July 2007
  • IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/06/14/Hao Wang
    Plate 1 1H Psb1A2 RBS strong rbs BBa_B0031 Kit Plate 1 2G Psb1A2 RBS Weak BBa_B0032 Kit Plate 1 2I Psb1A2 RBS Medium BBa_B0033 Kit Plate 1 2K Psb1A2 RBS...
    730 bytes (18 words) - 10:25, 16 August 2009
  • Endy:Screening plasmid/v1.0/Glycerol box 4
    <bbpart>I13575</bbpart> psb1a2 Cw2553/pJat8 <bbpart>I13571</bbpart> psb1a2 Cw2553/pJat8 <bbpart>I13574</bbpart> psb1a2 Cw2553/pJat8 <bbpart>I13507</bbpart> psb1a2 Cw2553/pJat8...
    893 bytes (0 words) - 14:31, 24 August 2007
  • IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/06/13/Hao Wang
    computer, search Parts: 16:55 dissolve the plasmids, prepare to add 15uL ddH2O transform 21:20 Transform plasmids above...
    786 bytes (28 words) - 10:25, 16 August 2009
  • Berk2006-Constructs
    BCH072006-Construction of pSB1A2-J23018 KAD072106-Construction of pSB1A2-J23029 KAD072106-Construction of pSB1A2-J23024 KAD072106-Construction of pSB1A2-J23025 SIL072506-Construction...
    6 KB (563 words) - 16:27, 27 November 2006
  • Berk2006-StockBoxes (section Box1)
    Box7: pSB3C6-J01122 KCM Competent cells (orange and green stripes). Box 8: TG1 Back Compartment: Olambda F plasmid (OriT knockout) KCM competent cells....
    5 KB (65 words) - 14:21, 22 January 2007
  • IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/06/24/Hao Wang
    BBa_R0062 09 Kit1 6O pSB1A2 Promoter BBa_R0065 09 Kit1 8C pSB1A2 Promoter BBa_R1062 09 Kit1 8G pSB1A2 Promoter...
    288 bytes (26 words) - 10:25, 16 August 2009
  • Berk2006-LocksAndKeysTeam
    (pJ23006-J23007-B0015 1 and 2, pJ23006-J23008-B0015 1 and 2, pSB1A2-J01129-B0015 1 and 2) sent pJ23006-J23009-1, pSB1A2-J01129-B0015-1, and pSB1A2-J01129-B0015-2 for sequencing...
    29 KB (4,932 words) - 11:48, 15 November 2006
  • Endy:Screening plasmid/v2.0/Glycerol box
    screening plasmid 2.0 1 I13545 psb1AT3 DH5a P1010 psb3K3 DB3.1 I13546 psb3K3 MC4100 I13547 psb3K3 MC4100 .......... I13549 psb3K3 MC4100 I13550 psb3K3 MC4100 I13551...
    1 KB (0 words) - 17:48, 21 June 2007
  • Berk2006-Sequencing
    SIL_080306 pSB1A2-J23038-1 miniprep ca998 failed read iG075 iG076 SIL_080306 pSB1A2-J23038-2 miniprep ca998 failed read iG076 iG077 SIL_080306 pSB1A2-J23028-3...
    32 KB (4 words) - 11:07, 14 December 2006
  • IGEM:IMPERIAL/2007/DNA Constructs
    promoter pSB1A2 AmpR High 937bp 3016bp Well 15H, Plate 2 500 A W G 05 BBa_R0040 tetracyclin promoter pSB1A2 AmpR High 54bp 2133bp Well 7O, Plate 1 60 A W...
    21 KB (0 words) - 03:21, 2 October 2007
  • Berk2006-ConjugationTeam
    sequencing. Picture of complements digested in EcoR1/Spe1 (pSB1A2-J23013 (1), pSB1A2-J23013(2), pSB1A2-J23011, ladder) We are also almost out of TriK plates...
    32 KB (4,682 words) - 11:57, 21 July 2006
  • Arking:JCAOligoTutorial1b
    ca1067F ca1067R pSB1AK3-b0015 pcrpdt Digest pcrpdt EcoRI,SpeI 1 pcrdig Digest pSB1A2-I13521 EcoRI,SpeI 1 vectdig Ligate pcrdig vectdig pSB1A2-Bca9128 oligo...
    41 KB (4,103 words) - 13:08, 16 January 2024
  • IGEM:Harvard/2006/Lab Intro
    miniprep pSB1AT3 Digest - E + P (construction) R0010 Digest - E + S (part 1: prefix) plasmid pSB1A2 (amp resistance) E0241 Digest - X + P (part 2: suffix)...
    5 KB (607 words) - 05:42, 14 June 2006
  • Berk2006-Samantha's Notebook (section JCAnderson 22:38, 2 October 2006 (EDT))
    pSB1AK3-J23074 (ES) pJ23019 (ES) pSB1A2-J23075 (ES) pSB1AK3-J23074 (AvrII/AlwNI) pSB1A2-J23075 (AvrII/SpeI) The J23074 clone 1 is correct, and I cut the slices to...
    16 KB (2,433 words) - 15:40, 11 December 2006
  • IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/14 (redirect from IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/14 2)
    wt col1 1.96 2.35 340.1 attP HK wt col2 1.92 2.32 411.5 attP HK mut col1 1,92 2.29 329.1 attP HK mut col2 1.97 2.30 282.7 attP λ mut col1 1.95 2.24 282...
    4 KB (0 words) - 19:32, 26 September 2017
  • Endy:Dedicated systems/Virtual machines (section VM1.0)
    recognized by ribosomes with a specific mutation in the 16s rRNA. Currently on pSB1A2. Slightly modified from Bacterial cell culture. Streak a fresh plate. Either...
    7 KB (926 words) - 22:00, 30 May 2006
  • Chassis/System - BC Box 1
    E7108.1AK3.BL21(VM1.1) 2/18 2.8mCherry.E7108.1AK3.BL21(VM1.1) 2/18 4B.mCherry.E71205.1AK3.BL21(VM1.1) 2/18 8mCherry.E71205.1AK3.BL21(VM1.1) 2/18 2.8mCherry...
    11 KB (0 words) - 11:22, 27 August 2008
  • Archive -80F
    Vector_pSB3K3 R0065.B0034.LacZ Strain_Omnimax (Invirtogen) BBa_I15030 Vector_pSB1A2 Strain_Top10F’ (?) BBa_I15030 Vector_pSB3K3 BBa_J13203 Vector_pSB4A3 PASK-IBA3C...
    3 KB (0 words) - 14:58, 28 July 2006
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