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- 6.Prepare PEI mix in 4 pieces of eppendorf tubes: A. Per 1 well: PEI μL 1.3 NaCL μL 25 B. Per 16 well: PEI μL 20.8 NaCL μL 400 vortex the PEI- NaCl mixes...5 KB (894 words) - 10:06, 27 September 2010
- Prepare PEI mix: add 1,3 µl PEI solution to 25 µl 150 mM NaCl/well, then vortex briefly. 8. Add PEI mix to DNA mixes in drops: 25 µl PEI mix 25 µl...26 KB (757 words) - 11:58, 14 July 2010
- media used depends on the type of the cell line. II. PEI solution PEI is used for PEI-mediated transfection, we need a 10 mM solution of it. I. The Media For...3 KB (528 words) - 10:22, 27 September 2010
- PEI 1. Dissolve 50 mg PEI (Polysciences 23966) in 50 mL ddH20. Heat at 55C for at least 30 minutes until dissolved and clear. 2. Adjust pH to 7 with HCl...1 KB (233 words) - 13:42, 30 April 2009
- Removal of the transfection medium The goal is to get rid of the transfection medium from the COS1 cells 5-7 hours after transfection, because: - PEI can damage...7 KB (1,098 words) - 10:16, 27 September 2010
- disulfide-crosslinked polyethyleneimine (PEI), and plasmid DNA was relatively non-toxic and achieved significantly higher transfection efficiency than other polymer...17 KB (25 words) - 23:38, 2 March 2018
- (spaces 3-8 in 5th row, yellow tier). Heather 11:47, 25 May 2009 (EDT): Transfection (HEK) 40' complex incubated, rather than the 30' recommended. GFP = 150...2 KB (0 words) - 17:03, 26 September 2017
- Thompson, D. B. & Liu, D. R. Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins. Proceedings of the National Academy...10 KB (2,247 words) - 20:37, 27 October 2012
- Biosystems Automatic Synthesizer) [92]. A strong paper, with luciferase transfection assay, in situ flourescent hybridization to detect plasmid dna, and a...60 KB (9,974 words) - 09:23, 12 July 2014
