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  • samples sink in the well). Error creating thumbnail: File missing Ficoll sucrose glycerol *sieving agarose The precise amount of dye is not important. However...
    4 KB (407 words) - 14:37, 24 February 2016
  • solutions MS plates for plant growth MS plates for plant growth (buffered, no sucrose) Exo-SAP PCR cleanup for sequencing Seed Storage Eleven Golden Rules of...
    5 KB (8 words) - 00:19, 2 December 2016
  • 0.5M Sucrose, 0.1M MgCl2 Species = L. fermentum Dilution = 1/50 Growth = 1% Glycine, 37°C Wash = 5mM NaH2PO4 1mM MgCl2 (0°C) Buffer = 0.9M Sucrose 3mM MgCl2...
    8 KB (1,217 words) - 21:31, 16 September 2011
  • (Solanum tuberosum) displaying alterations in cellular or extracellular sucrose metabolism. Metabolomics 4:161-170. Farre EM, Tech S, Trethewey RN, Fernie...
    6 KB (7 words) - 13:30, 19 June 2017
  • gradient of 15% sucrose until tissue sinks, then 25% sucrose until tissue sinks, then 30% sucrose until tissue sinks. Sucrose cryopreservative (antifreeze)...
    18 KB (2,571 words) - 22:01, 27 February 2023
  • [show][hide] Formula: [10 mM HEPES (pH 7.9); 10 mM KCl, 1.5 mM MgCl2; 0.34 M sucrose; 10% glycerol; 1 mM dithiothreitol; 1x protease inhibitor cocktail] Volume:...
    10 KB (1,206 words) - 20:51, 9 December 2015
  • Buffer (0.5M Sucrose, 10% glycerol). 7. Centrifuge cells for 15 mins at 3000g. 8. Resuspend pellet in 3ml Electroporation Buffer (0.5M Sucrose, 10% glycerol)...
    1 KB (195 words) - 19:06, 6 April 2011
  • solution 1, 3x wash volume so ~30ml R1: Tris-HCl + 0.5M sucrose, 30ml EDTA, 0.1M, 3.3ml SMM = sucrose, 0.5M , , 30 + 3x wash volume, so ~60ml W2: SMM for washing...
    3 KB (525 words) - 15:09, 4 April 2007
  • 3 g agar + 250 mL broth per bottle Autoclave 1 L dH2O 20 g peptone 20 g sucrose 5 g yeast extract 12 g agar (optional) 3 g agar + 250 mL broth per bottle...
    6 KB (660 words) - 15:54, 16 August 2013
  • MRS media Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6) Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino...
    4 KB (529 words) - 19:03, 6 April 2011
  • Cryoprotection: Cryoprotected in 30% Sucrose in 0.1M PBS Material label and storage: Labeled as RC-001 and stored in sucrose in a vial prior to being cut. The...
    4 KB (342 words) - 02:15, 10 September 2013
  • solution of MgCl2 Stock Solution of Glucose Electroporation Buffer (0.5M Sucrose, 10% Glycerol) Grow cells overnight in MRS Broth at 30°C. Use overnight...
    2 KB (262 words) - 01:13, 17 September 2011
  • leaving the embryo in 0.1M PB 30% Sucrose solution overnight. Material label and storage: Labeled as MH001 and stored in a sucrose filled vial. Objective: Practiced...
    2 KB (223 words) - 02:14, 10 September 2013
  • the counter) HEPES/Sucrose Effects on deGFP Expression Zoila Jurado (zjuradoq at caltech dot edu) Fall 2018 E. Coli S30 HEPES/Sucrose buffer negativity...
    4 KB (266 words) - 21:28, 11 February 2019
  • carbohydrates, looking in particular at the difference between glucose and sucrose. If sucrose is added to YEP medium and glucose is reduced, the yeast must derepress...
    18 KB (2,880 words) - 18:12, 13 May 2010
  • carbohydrates, looking in particular at the difference between glucose and sucrose. If sucrose is added to YEP medium and glucose is reduced, the yeast must derepress...
    18 KB (2,986 words) - 15:07, 6 December 2010
  • carbohydrates, looking in particular at the difference between glucose and sucrose. If sucrose is added to YEP medium and glucose is reduced, the yeast must derepress...
    18 KB (2,958 words) - 18:28, 21 April 2011
  • carbohydrates, looking in particular at the difference between glucose and sucrose. If sucrose is added to YEP medium and glucose is reduced, the yeast must derepress...
    18 KB (2,982 words) - 18:37, 15 August 2011
  • Maloof, J. N., and Nemhauser, J. L. (2011). PIF genes mediate the effect of sucrose on seedling growth dynamics. PLoS ONE 6, e19894. [11] Kerwin, R. E., Jimenez-Gomez...
    10 KB (8 words) - 05:49, 3 February 2013
  • possible parts list from Jean @ Olin New lab for 2012: cyanobacteria, sucrose production (w/ Silver lab), Paper from Danny Ducat...
    1 KB (135 words) - 13:59, 13 December 2011
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