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- under tetracyclin promoter pSB1A2 AmpR High 937bp 3016bp Well 15H, Plate 2 500 A W G 05 BBa_R0040 tetracyclin promoter pSB1A2 AmpR High 54bp 2133bp Well...21 KB (0 words) - 10:21, 2 October 2007
- be incorporated into a vector. The vector will be made from the plasmid pSB1A2 [2] or pSB2K3 [3] and the promotor BBa_R0080 [4] or BBa_I0500 [5]. These...5 KB (0 words) - 19:03, 23 September 2017
- Endy:Screening plasmid/Inverter characterization (section Measured with older versions of screening plasmid (I13537.pSB1A2, I13538.pSB1A2, or I13534.pSB4A3))Q04400.007 - mutated Q04400 (tetR QPI), point mutation in RBS. Works well, switches later than Q04400. mnt inverters from enterobacteriophage p22 [33,...4 KB (429 words) - 03:38, 27 July 2006
- BBa_I719005 T7 Promoter [[5]] Physical DNA: Library: Spring 2008 Well: 3G Plasmid: pSB1A2 Source Plate: 1006 * BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage)...8 KB (0 words) - 19:03, 23 September 2017
- what the vector names mean. constructed by Tom Knight (formerly known as pSB1A2-1) derived from pSB1A1 constructed by Leon Chan (formerly known as pSB1A3-1...5 KB (626 words) - 19:14, 25 July 2007
- <bbpart>E0240</bbpart> pSB1A2 MG1655 R0040 <bbpart>I13401</bbpart> pSB1A2 MG1655 <bbpart>J04650</bbpart> pSB1AK3 MG1655 <bbpart>B0032</bbpart> pSB1A2 MG1655 <bbpart>P1010</bbpart>...2 KB (0 words) - 13:50, 29 August 2007
- BioBrick assembly scheme for BioBricks on pSB1A2 plasmids. See details below. Part A (blue) and Part B (red) are on pSB1A2 plasmids encoding ampicillin resistance...8 KB (1,135 words) - 07:19, 26 May 2009
- PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp Miniprep sizes expected: 2613 bp & 2079 bp (pSB1A2) Status: Completed & Sequenced <bbpart>F2620</bbpart>...6 KB (741 words) - 11:07, 25 September 2006
- is currently available in Biobricks Registry: BBa_E7104 (available), in pSB1A2 (AmpR) pT7 can be used with T7 RNAP in vitro and in veso, and it gives constitutive...8 KB (536 words) - 10:12, 21 August 2007
- plasmid that contained a promoter was decided to be used instead. The plasmid pSB1A2 with promoter BBa_R0040, BBa_R0051, BBa_R001 was extracted from IGEM 2007...10 KB (0 words) - 19:03, 23 September 2017
- pSpoIIE kan pSB1A2-I13522 in JM109 pSB1A2-S03155 994 Library 06/27/06 pSB1A2-T9002 in TGI pSB1A2-P0412 in TGI pSB1A2-I13603 in TGI pSB1A2-F2620 in TGI...5 KB (65 words) - 21:21, 22 January 2007
- <bbpart>I13434</bbpart> psb1A2 CW2553/pJat8 <bbpart>I13521</bbpart> psb1a2 Cw2553/pJat8 <bbpart>I13573</bbpart> psb1a2 Cw2553/pJat8 <bbpart>I13575</bbpart> psb1a2 Cw2553/pJat8...893 bytes (0 words) - 21:31, 24 August 2007
- Plate 1 1H Psb1A2 RBS strong rbs BBa_B0031 Kit Plate 1 2G Psb1A2 RBS Weak BBa_B0032 Kit Plate 1 2I Psb1A2 RBS Medium BBa_B0033 Kit Plate 1 2K Psb1A2 RBS Weaker...730 bytes (18 words) - 17:25, 16 August 2009
- (<bbpart>B0015</bbpart>) E0240 (pSB1A2) B0032 (pSB1A2) + J04650 (pSB1AK3) R0040 (pSB1A2) + I13401 (pSB1A2) R0040 (pSB1A2) + J04650 (pSB1AK3) 1 - <bbpart>E0240</bbpart>...2 KB (194 words) - 18:46, 15 June 2007
- [RA2 Promoter][RBS] Digest pSB1A2-RA2 (pSB1A2-J23104) from 1022 library (SpeI/AlwNI/PstI, Neb2, Large 1568 ) Paste into pSB1A2-B0034 (XbaI/AlwNI, Neb2, Small...204 bytes (30 words) - 18:23, 7 August 2006
- [OriT]-[key3] digest pSB1A2-J23070 (Spe,AlwN1) digest pSB1A2-J01003 (Xba, AlwN1) ligate, product is pSB1A2-J23121...133 bytes (17 words) - 20:11, 8 October 2006
- dblTerm] digest pSB1A2-J01052 (SpeI/AlwNI, Neb2, Large-2241) digest pSB1A2-B0015 (XbaI/AlwNI, Neb2, Small-546) Ligate, Product pSB1A2-J23000...197 bytes (25 words) - 19:14, 22 June 2006
- dblTerm] digest pSB1A2-J01001 (SpeI/AlwNI, Neb2, Large-1905) digest pSB1A2-B0015 (XbaI/AlwNI, Neb2, Small-546) Ligate, Product pSB1A2-J23003...189 bytes (24 words) - 19:20, 22 June 2006
- [pK2 (presumably on pSB1A2)]+[J23008] Digest pJ23006-J23008 (XbaI/AlwnI, neb2, small-648bp) Paste into pSB1A2-J01007 (SpeI/AlwnI, neb2, large-1593bp) Product...186 bytes (26 words) - 21:25, 3 August 2006
- [pK3 (presumably on pSB1A2)]+[J23008] Digest pJ23006-J23008 (XbaI/AlwnI, neb2, small-648bp) Paste into pSB1A2-J01006 (SpeI/AlwnI, neb2, large-1593bp) Product...186 bytes (26 words) - 21:26, 3 August 2006
