Search results
From OpenWetWare
Jump to navigationJump to search
- cells in 37 degree C incubator labeled with M.A.N.G, date and time. Wed (4/6): Nikit comes in at 9pm pick a colony and culture it in 3ml LB (tell GSI)....3 KB (633 words) - 13:09, 11 April 2011
- E, G, H, R, T, A2, J, K, L, M, N, tos, O) that maps onto: sbb35 sbb06 sbb01 sbb20 sbb21 Bca1745 sbb14 sbb04 sbb36 sbb37 sbb39 sbb40 sbb41 sbb03 sbb23 Also...3 KB (394 words) - 11:41, 25 March 2010
- plate +Spec, pick colony, grow in 2mL liquid in a 10mL culture tube in LB +Spec overnight at 37C {O/N Toxic} 1. Thaw a 200 uL aliquot of competent cells...5 KB (956 words) - 10:22, 28 April 2011
- pBca1766 jtk2979 pBjh1601KC sbb1117 pBjh1601KC sbb1129 pBjh1601KC sbb1105 pBjh1601KC sbb1110 pBjh1601KC sbb1139 AB1 pBjh1601KC-sbb1117- Perfect BB1- Perfect...7 KB (1,145 words) - 14:31, 2 May 2011
- Native N-terminus - OmpX {N.ompX!} PCR VU015F/VU016R on OmpX (529 bp, EcoRI/BamHI) Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, 2057+910, L) Product is M10012...16 KB (2,153 words) - 13:04, 19 May 2009
- they will be at a concentration of 28.80 nmol (may be at a diff concentration, need to check) and we want a final concentration of 100uM --> add 288 ul...16 KB (2,689 words) - 22:58, 6 March 2009
- from each test tube onto a plate to go into a Tecan plate reader- Do this twice per test tube so that all samples are duplicates. a.) Setup Tecan to measure...13 KB (1,969 words) - 01:18, 3 May 2011
- plate +Spec, pick colony, grow in 2mL liquid in a 10mL culture tube in LB +Spec overnight at 37C {O/N Toxic} 1. Thaw a 200 uL aliquot of competent cells...17 KB (2,505 words) - 10:42, 3 May 2011
- ==PCR for part sbb1217== 1. ca998/osbb1333R on template pBca9145-jtk2768 a)osbb1333R oligo is 34.6nM, MW=11,944.7, Tm=72.1°C. I prepared a dilute solution...20 KB (3,563 words) - 12:46, 5 April 2012
- SBB12Ntbk-AuDuong (section For part sbb1229)2.5mL of Media 100nM: 2.0mL of 250nM + 3.0mL of Media 50nM: 2.5mL of 250nM + 2.5mL of Media 10nM: 1.0mL of 250nM + 4.0mL of Media 1nM: 500uL of 250nM +...47 KB (7,733 words) - 04:41, 12 May 2012
- collected product into a fresh Eppendorf tube. 3. (Skip running on a gel due to smeary mess) Conduct amplification after zymo cleanup: a)label a PCR tube "PCA2...29 KB (4,792 words) - 21:32, 1 May 2012
- Violacein is a chromophore and makes cells look purple. That suggests that nothing was mislabeled. So we proceded. At ~5PM today, Jessica started O/N cultures...12 KB (1,742 words) - 15:31, 1 May 2011
- dropped a c 370-402 - perfect match c instead of tt 404-420 - perfect match g instead of a 422-555- perfect match Today I looked at sbb0083. This was a little...12 KB (2,300 words) - 13:30, 25 March 2009
- Janelle N. Ruiz Assignment 5 (11) SBB10IndivCombined (11) Amanda N. Wavrin Week 4 (11) Alex J. George Week 4 (11) Alex J. George Week 6 (11) SBB10Ntbk-JingLuo...95 KB (7,819 words) - 10:24, 3 October 2020
- 100uM by adding 10X nmol. PCR of M10006 (<N.CPG_L2!, regular part, 211bp), A-M10007 (371bp), B-M10007 (343bp), A-M10009 (548bp), B-M10009 (175bp), M10010...10 KB (1,181 words) - 17:03, 14 May 2009
- SBB13Ntbk-SeeYoungLee (section April 11, 2013)Colonies 1. Add 5mL of LB media with ampicilin to a clean test tube. 2. Pick a well-isolated, round, and "normal" looking colony with a micropipette tip...14 KB (2,274 words) - 02:32, 16 May 2013
- Protein A Flag Concurrent notes about my project- My project is to find a engineered protein A. Protein A is an antigen which has been known to bind to...12 KB (2,117 words) - 09:14, 11 May 2009
- result in 0 deg C. Around a quarter of a cup of ice was added to a bucket an inch deep in water. A thermometer was left to ensure a constant temperature. The...18 KB (2,898 words) - 17:24, 30 April 2011
- another single nucleotide change from a to g at 1198. The missense mutation changes the a.a. from Asp to Gly. 50-700 usually good read Original EhaB autotransporter...24 KB (3,646 words) - 08:34, 8 May 2009
- Redo sbb13 (as I predicted), depending on what JCA says. sbb11-1, sbb11-2, sbb11-3, sbb11-4 - 4 clones picked from sbb11 plate sbb13-1, sbb13-2, sbb13-3...31 KB (4,782 words) - 11:30, 29 April 2010
