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- transform a bacterial culture with a plasmid or whenever you obtain a new bacterial strain you will want to make a long term stock of that bacteria. Bacteria...2 KB (254 words) - 01:38, 28 June 2012
- Transforming chemically competent cells Bacterial transformation Making a long term stock of bacteria P1vir phage transduction Preparing chemically competent cells...10 KB (12 words) - 18:01, 31 August 2017
- Transforming chemically competent cells Bacterial transformation Making a long term stock of bacteria P1vir phage transduction Preparing chemically competent cells...2 KB (474 words) - 12:22, 8 April 2008
- Back to Making a long term stock of bacteria 40% glycerol solution Cryogenic vials Add 1 ml of 40% glycerol in H2O to a cryogenic vial. Add 1 ml sample...821 bytes (138 words) - 09:40, 29 September 2006
- electrocompetent cells β-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Pouring plates...731 bytes (47 words) - 10:25, 20 June 2006
- chemically competent cells beta-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Recombineering...7 KB (4 words) - 11:37, 28 July 2006
- Freezing cells (Protocol from OWW: Making a long term stock of bacteria) PCR v. miniprep (flow chart) <html> <!-- Start of StatCounter Code --> <script ty...2 KB (144 words) - 09:50, 30 October 2006
- PCR 1 kb Plus DNA Ladder Freezing cells (Protocol from OWW: Making a long term stock of bacteria) PCR v. miniprep (flow chart) T9002/J37016/J37020 Testing...2 KB (109 words) - 03:48, 4 September 2006
- electrocompetent cells β-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Pour LB plates...5 KB (4 words) - 18:09, 11 September 2007
- chemically competent cells beta-Galactosidase Assay (A better Miller) Colony PCR Making a long term stock of bacteria Miniprep P1vir phage transduction Recombineering...7 KB (4 words) - 12:24, 21 August 2006
- transformation Endy:Yeast Colony PCR Making a long term stock of bacteria P1vir phage transduction Silver staining Purification of His-tagged proteins FBA MoMA...1 KB (7,336 words) - 14:49, 17 May 2007
- g_term_stock_of_bacteria Method: Add 1 ml of 40% glycerol in H2O to a cryogenic vial. Add 1 ml sample from the culture of bacteria to be stored. Gently...4 KB (640 words) - 07:30, 6 July 2007
- below: Endy:Making_a_long_term_stock_of_bacteria: these are glycerol stocks for the -80ºC freezer, used so that you can store a cell culture for a much later...3 KB (456 words) - 07:36, 12 April 2011
- Kit. Making glycerol stock for long-term storage Follow steps 1-4 under "Recovering plasmid DNA". The optimal concentration of glycerol for long-term storage...4 KB (665 words) - 14:26, 20 August 2009
- P1vir phage transduction (1) PCR techniques (1) Making a long term stock of bacteria (22) Institute of Biological Engineering (17) JHIBRG (12) IGEM (12)...195 KB (15,593 words) - 21:50, 24 August 2010
- BE.109:Protein engineering/Tools for protein engineering (section Part 4: Induce expression of your modified beta-galactosidase)presence of the unnatural amino acid you’ve selected based on your study of the structure of the molecule. You’ll be provided with a sample of bacteria growing...21 KB (3,213 words) - 17:26, 14 March 2006
- P1vir phage transduction (2) Bacterial transformation (2) Making a long term stock of bacteria (2) Silver staining (2) Transforming chemically competent...179 KB (13,843 words) - 10:24, 3 October 2020
- Protocol: Making glycerol stock for long term storage: 1. Add 1 ml of 40% glycerol in H2O to a cryogenic vial. 2. Add 1 ml sample from the culture of bacteria...1 KB (178 words) - 18:41, 8 June 2007
- Transforming chemically competent cells (1) Maxiprep of plasmid DNA from E. coli (1) Making a long term stock of bacteria (1) Miniprep/Kit-free high-throughput protocol...196 KB (15,771 words) - 23:00, 24 August 2010
- E. coli is a typical bacteria used for molecular cloning. Long-term storage of BioBrick plasmids is in E. coli suspended in glycerol (500ul cells:500ul...843 bytes (149 words) - 08:10, 7 June 2010
