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- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/20 (category OWWLabNotebookV1)4 KB (0 words) - 21:53, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/03 (category OWWLabNotebookV1)= pSG.021 pSG.003 (S, P) + pSG.020 (X, P; ColE7 Imm) = pSG.022 Incubate at 20°C (microscope room) for 1h No colonies because I did not purify the immunity...3 KB (0 words) - 21:51, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/06 (category OWWLabNotebookV1)microscope room (20°C) only 1 colony, on the (-) control plate :( Grow cells Wild type ColE2 ColE7 overnight (saturated) Dilute 100x; plate 20uL with beads...6 KB (0 words) - 21:52, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/29 (category OWWLabNotebookV1)entry Next entry Prepared competent NEB Turbo+pSG.008 (ColE2 Immune) Prepared Z1.08.23 cells +-IPTG and WT ColE2 for toxicity assay Digested pSG.013-1, pSG.013-2...7 KB (0 words) - 21:55, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/28 (category OWWLabNotebookV1)Plate Lawn (20 uL) Spot (5uL) LB MG1655 MG1655 LB MG1655 WT ColE2 LB MG1655 Z1 pSG.008 WT...8 KB (0 words) - 21:55, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/16 (category OWWLabNotebookV1)ligated pTet (annealing product) with pSG.002 (E,S), Kan resistant Incubate in 20°C (microscope room) for 1h Protocol...10 KB (0 words) - 21:53, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/07 (category OWWLabNotebookV1)type of cells: Wild type MG1655 Turbo MAGE Top 10 were diluted 100x, plated 20 uL with beads then 5 uL drops of ColE2 ColE7 the same type of cell added to...9 KB (0 words) - 21:52, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/09 (category OWWLabNotebookV1)liquid culture The saturated cultures were diluted 100x in LB, then plated (20uL) Drops of saturated ColE2 cells (5uL) were added to each plate With the...7 KB (0 words) - 21:53, 26 September 2017