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- 202 bytes (0 words) - 12:21, 15 October 2012
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/25 (category OWWLabNotebookV1)1,017 bytes (0 words) - 22:09, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/15 (category OWWLabNotebookV1)1 KB (0 words) - 22:07, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/24 (category OWWLabNotebookV1)1 KB (0 words) - 22:08, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/23 (category OWWLabNotebookV1)grow in 3 differents tubes of 10mL of LB+10µL of Cm+10µL of Amp. I also made culture of supD+KanR (same condition with 10µL of Kan) from the 3 -80°C tubes...1 KB (0 words) - 22:08, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/16 (category OWWLabNotebookV1)taq, dNTP, ..) PCR pgm : 95°C - 1' 95°C - 30" 50°C - 30" 72°C - 1' 72°C - 10' 10°C - inf -> 30 cycles Pictures of the gels :...2 KB (0 words) - 22:07, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/22 (category OWWLabNotebookV1)2 KB (0 words) - 22:08, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/29 (category OWWLabNotebookV1)is : 95°C - 30" 95°C - 30"--- ı 55°C - 1' x 12 times 68°C - 4' --- ı 68°C - 10' 10°C - [math]\displaystyle{ inf }[/math] for the control, I strictly follow...2 KB (0 words) - 21:55, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/20 (category OWWLabNotebookV1)project page Previous entry Next entry pSC001 : 1/1 : +++ 1/10 : ++ pSC003 : 1/1 : ~ 13 1/10 : 3 LB alone : 0 NEB alone : 0 pSC001 is also a positive control...2 KB (0 words) - 21:44, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/16 (category OWWLabNotebookV1)plate. It was in NEB turbo, pSB1A2, on Ampicillin. Make it grow in 10mL of LB + 10 µL Amp (1000X) 37°C O/N...1 KB (0 words) - 21:44, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/24 (category OWWLabNotebookV1)Here is the content of 1 tube of PCR : VR (diluted 10 times from the stock tube) : 2,5µL VF2 (diluted 10 times from the stock tube) : 2,5µL PCR MM 2X : 25...2 KB (0 words) - 21:47, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/26 (category OWWLabNotebookV1)(µL) Plates (LB + Cm) C1 20 40 180µL pur & 200µL 1/10 & 200µL 1/100 C2 20 40 180µL pur & 200µL 1/10 & 200µL 1/100 positive transformation control (pSB1C3)...3 KB (0 words) - 21:54, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/22 (category OWWLabNotebookV1)efficient. Anyway, I made 2 clones in culture at 10:30 am. The culture is 10mL of LB + 10µL of Kan and 10µL of Amp. I'll miniprep this culture around 5 pm...2 KB (0 words) - 21:54, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/02 (category OWWLabNotebookV1)What's in the tube DNA volume (µL) Cell volume (µL) Plates L1 10 20 200µL pur Cm L2 10 20 200µL pur Cm L3 10 20 200µL pur...4 KB (0 words) - 21:56, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/27 (category OWWLabNotebookV1)volume (µL) Plates (LB + Cm) C1 20 40 190µL pur & 100µL 1/10 C2 20 40 190µL pur & 100µL 1/10 positive transformation control (pSB1C3) 0,5 20 200µL negative...3 KB (0 words) - 21:54, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/23 (category OWWLabNotebookV1)the ligation : vector (pSC001) : 1 µL (83ng) insert (L11 or L12) : 10 µL buffer 10X : 2 µL T4 dna ligase : 1 µL water : 6 µL I also ligate the insert or...3 KB (0 words) - 21:46, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/22 (category OWWLabNotebookV1)New name BBa_I14034 P(Kat) 2012/2/13B pSB2K3 pSC004 BBa_B0034 RBS 2012/1/2M pSB1A2 pSC005 BBa_B0015 double terminator 2012/1/23L pSB1AK3 pSC006...4 KB (0 words) - 21:45, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/21 (category OWWLabNotebookV1)tube DNA volume (µL) Cell volume (µL) Plates (LB + Amp) Ligation 10 40 180µL pur & 200µL 1/10 positive transformation control (pSB1A2::R0011) 0,5 20 200µL...3 KB (0 words) - 21:45, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/20 (category OWWLabNotebookV1)Here is the content of 1 tube of PCR : VR (diluted 10 times from the stock tube) : 2,5µL VF2 (diluted 10 times from the stock tube) : 2,5µL PCR MM 2X : 25...4 KB (0 words) - 22:01, 26 September 2017
- IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/30 (category OWWLabNotebookV1)afternoon. I make them grow in 10ml of LB+Amp and in LB+Kan On the pUC control, there are about 20 colonies I run a gel with 10µL of the digested DNA to check...1 KB (0 words) - 21:56, 26 September 2017