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- wavelength. You will be designing and then creating an expression vector to delete the first 32 amino acids of EGFP. Cells transfected with your expression...4 KB (396 words) - 10:32, 10 October 2007
- Openwetware([[1]]). In protocols Guanidinium Thioyanate(W.M. 118.2) final concentration is 0.8M, but 118.2g is used in 1L Trizol. Ammonium thiocyanate (W.M. 76.1)...6 KB (1,056 words) - 02:13, 18 March 2015
- or delete sections as appropriate. Replace this sentence with a brief description of the protocol and its goal. For a 50 μL PCR reaction: 35 μL H2O 5 μL...3 KB (304 words) - 14:27, 18 June 2012
- T7.2 is part of a larger project of Rebuilding T7 to construct a more understandable model organism. T7.2 is the second iteration of our work in creating...5 KB (765 words) - 17:04, 28 March 2006
- attending a scan should be Level 1 or 2 trained unless they are the participant. Every scan needs at least one Level 2 trained operator to be present for...6 KB (12 words) - 10:59, 2 February 2024
- Read in the NMRPipe-format 2D fid. Process the first increment Edit the script “process2d.com” Execute the script “process2d.com” Read the spectrum “test...4 KB (357 words) - 21:31, 2 January 2006
- replace the text in this page with your own protocol. Feel free to add or delete sections as appropriate. Add the primary curators here (including their...4 KB (463 words) - 11:38, 17 June 2009
- restriction system (we don't want our DNA cut up randomly on transformation) Delete the methylation specific restriction systems encoded by the mcrA, mcrBC...20 KB (3,334 words) - 21:42, 1 January 2009
- make a large bacterial liquid culture 2 strains of phage were used in these experiments: (1) T7+ (wild-type) (2) T7.1 alpha-beta phage titering was used...7 KB (1,101 words) - 13:07, 23 January 2006
- severely as to prevent efficient separation (e.g., the genes 4A, 4B, 4.1, and 4.2). Finally, some functional genetic elements were very short (<150bp) such that...7 KB (1,098 words) - 19:54, 27 April 2006
- recombination (x2) Dedicated RNA & protein systems: (efficiency >10%, crosstalk <10%) Virtual Machines & Demand specifications Delete phage lysogens &...3 KB (308 words) - 05:37, 9 July 2008
- T7.2/Design (section T7.2 Alpha Design)-- Phi2.5 -- A1,A2,A3,BoxA -- R0.3 -- 0.3 -- R0.3 -- 0.7 -- R0.3 -- 1 -- ORI -- 1.1 -- 1.2 -- phi 3.8 -- R0.3 -- 1.3 -- TE -- phi2.5 -- 1.7 -- 2 -- phi2...9 KB (1,269 words) - 12:43, 30 May 2006
- BME100 f2017:Group6 W1030 L6 delete this page (section Feature 2: Hardware - PCR Machine & Fluorimeter)BME 100 Fall 2017 Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors...10 KB (0 words) - 22:12, 21 November 2017
- only 'solution' is to delete the large log file, delete the temporary files, and restart the computer. It's not enough to delete the cab files because...8 KB (1,034 words) - 16:48, 21 November 2017
- cells (2). At low concentrations of arabinose, degradation of the sugar within cells also effects the homogeneity of expression from PBAD promoters (2). Arabinose...6 KB (1,354 words) - 10:24, 23 January 2008
- protein in SAGA is needed for the yeast to survive. In this experiment we will delete one of the non-essential SAGA-subunits from the yeast genome and then ask...2 KB (276 words) - 04:13, 27 November 2006
- your Athena home directory): 1. Obtain Kerberos tickets $ kinit mitusername 2. Obtain AFS tokens $ aklog mitusername 3. Attach your Athena home directory...3 KB (410 words) - 13:44, 14 September 2008
- Primer 1 ul D32N-rev (=100 pmol) PCR Master Mix* 20 ul of 2.5X stock (see REAGENTS LIST) H2O to final volume of 50 ul The PCR Master Mix contains buffer...26 KB (3,501 words) - 17:58, 9 February 2006
- for first time use. 1) Make sure you are logged into dropbox in your browser 2) Follow this link to download the appropriate desktop version of rclone(usually...4 KB (506 words) - 10:50, 14 May 2023
- Disk 1TB $740, 2TB $1,050 (June 2007) Rack format Powered by Windows XP® Embedded Gigabit Ethernet LaCie Ethernet Disk RAID 1TB $840, 2TB $1,160, 4TB $3...10 KB (1,237 words) - 13:54, 5 December 2007