Shreffler:Notebook/Basophil Imaging

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Contents

Overview

Nanotubles

  • Reproduce
  • Structure
    • DNase
    • Actin
  • Time lapse
  • Function
    • depolarization (Ca flux)
    • intercellular transport
  • Activation dependence

Team

  • Alex Praslick
  • Matthew Gross
  • Madhan Masilimani
  • Wayne Shreffler

Meeting Notes

23 June

Alex and I met to discuss priorities for the summer.

  • Reproducibility of activation-dependent putative nanotubes
  • Characterization
    • Physical dimensions
    • Polarization
    • Actin: phalloidin
    • DNA: intercalating dyes, DNase treatment -- consult reference [1]
  • time lapse experiments

To Do:

  • order phalloidin
  • order mAbs
  • order new DAPI
  • review and decide on protocols for phalloidin staining as well as DNA staining/ digestion

Future Projects

Ag uptake

  • IgE-dependence
  • Traffic
  • Interaction with T cells

Activation-induced morphological changes

  • correlation of changes with flow
  • non-releaser phenotype
  • polarization
  • phosphorylation events
  • impact on intercellular activation?

References

Error fetching PMID 18516038:
Error fetching PMID 894382:
Error fetching PMID 15970657:
Error fetching PMID 18400194:
Error fetching PMID 15916720:
Error fetching PMID 15634893:
Error fetching PMID 15314076:
Error fetching PMID 17056503:
Error fetching PMID 18300366:
Error fetching PMID 17698921:
Error fetching PMID 18690244:
Error fetching PMID 19609275:
  1. Error fetching PMID 18690244: [Yousefi_2008]
  2. Error fetching PMID 18516038: [Denzel_2008]
  3. Error fetching PMID 894382: [Dvorak_2000]
  4. Error fetching PMID 15970657: [Dvorak_2005]
  5. Error fetching PMID 18400194: [Galli_2008]
  6. Error fetching PMID 15916720: [Hennersdorf_2005]
  7. Error fetching PMID 15634893: [Mack_2005]
  8. Error fetching PMID 15314076: [Min_2004]
  9. Error fetching PMID 17056503: [Shin_2006]
  10. Error fetching PMID 18300366: [Sokol_2008]
  11. Error fetching PMID 17698921: [Wu_2007]
  12. Error fetching PMID 19609275: [Yousefi_2009]
All Medline abstracts: PubMed HubMed
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